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用ＰＣＲ技术从纯化的乙型肝炎病毒核酸中扩增出ｐｒｅＳ２－Ｓ基因，并将其定向克隆于真核表达质粒ｐｃＤＮＡ３．１（＋）中，构建成带有完整的乙肝表面前Ｓ２抗原基因和Ｓ基因的重组质粒．重组质粒经酶切及部分序列分析鉴定后，瞬时转染小鼠Ｌ细胞，ＥＬＩＳＡ法检测到培养上清液有ＨＢｓＡｇ表达．用纯化的重组质粒静脉、肌肉和皮下注射免疫ＢＡＬＢ／ｃ小鼠，首次接种质粒ＤＮＡ３周后，血清抗体开始出现，再次加强２周后，阳性率明显提高．
The preS2-S gene was amplified from the purified hepatitis B virus nucleic acid by PCR and cloned into the eukaryotic expression plasmid pcDNA3.1 (+). The preS2-S gene was constructed with a complete preS2 antigen gene And S gene recombinant plasmid. The recombinant plasmids were transiently transfected into mouse L cells after being identified by restriction analysis and partial sequence analysis. HBsAg expression was detected by ELISA in the culture supernatant. BALB / c mice were immunized with the purified recombinant plasmids intravenously, intramuscularly and subcutaneously. After the first inoculation of the plasmid DNA for 3 weeks, the serum antibodies began to appear. After being boosted again for 2 weeks, the positive rate was significantly increased.