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目的：观察水芹乙酸乙酯提取物（简称水芹提取物）在乙型肝炎病毒（ＨＢＶ）基因转染的人肝癌细胞系（ＨｅｐＧ２）２２１５细胞培养中，对细胞的毒性及对ＨＢＶ表面抗原（ＨＢｓＡｇ）和ｅ抗原（ＨＢｅＡｇ）的抑制效果。方法：水芹提取物用培养液稀释成２０００、１０００、５００、２５０、１２５μｇ·ｍｌ－１浓度，分别加入２２１５细胞内培养１２ｄ，观察药物对细胞的毒性。在无毒浓度下的水芹提取物，以培养液稀释１０００、５００、２５０μｇ·ｍｌ－１３浓度，分别加入２２１５细胞内培养，于第６、９ｄ收集的培养液，用固相放射免疫法测定ＨＢｓＡｇ和ＨＢｅＡｇ，γ－计数器测定每孔药液ｃｐｍ值。结果：对细胞的半数中毒浓度（ＴＣ５０）平均为２２８４．７３±１２７．３５μｇ·ｍｌ－１，最大无毒浓度为１０００μｇ·ｍｌ－１。大、中剂量（１０００、５００μｇ·ｍｌ－１）对２２１５细胞分泌的ＨＢｓＡｇ、ＨＢｅＡｇ的活性于试验的第６、９ｄ均有明显的抑制作用（Ｐ＜０．０５，Ｐ＜０．０１，Ｐ＜０．００１），并有一定转阴作用。结论：水芹提取物在２２１５细胞中培养６～９ｄ，最大无毒浓度可明显抑制ＨＢｅＡｇ和ＨＢｓＡｇ的分泌。
Objective: To observe the toxicity of the ethyl acetate extract of cress (watercress extract) in the human hepatocellular carcinoma cell line (HepG2) 2215 transfected with the hepatitis B virus (HBV) gene and the toxicity to the cells and to the HBV surface antigen (HBsAg) and e antigen (HBeAg) inhibitory effect. Methods: The watercress extracts were diluted to 2000, 1000, 500, 250, and 125 μg·ml -1 with culture medium, and then added to 2215 cells for 12 days. The toxicity of the drugs to the cells was observed. The non-toxic concentration of the watercress extract was diluted to 1000, 500, 250 μg·ml -13 with the culture solution and cultured in 2215 cells. The culture fluid collected on the 6th and 9th days was measured by solid-phase radioimmunoassay. HBsAg and HBeAg, gamma counters determine the cpm value of each well. Results: The average half-toxic concentration (TC50) of the cells was 2284.73±127.35 μg·ml-1, and the maximum non-toxic concentration was 1000 μg·ml-1. The activity of HBsAg and HBeAg secreted by 2215 cells at large and medium doses (1000, 500 μg·ml-1) was significantly inhibited on the 6th and 9th day of the experiment (P<0.05, P<0.01, P). <0.001), and have a negative effect. Conclusion: The extracts of watercress were cultured in 2215 cells for 6 to 9 days. The maximum non-toxic concentration can significantly inhibit the secretion of HBeAg and HBsAg.