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目的 :建立HDV /HBV感染人胎肝细胞体外培养系统。方法 :利用HDV/HBV阳性血清同时感染体外培养的人胎肝细胞 ;应用ELISA、免疫组化法、原位杂交法和斑点法检测上清液和细胞中HBsAg、HDAg、HBVDNA和HDVcDNA。 结果 :上清液和细胞中HBsAg、HDAg、HBVDNA和HDVcDNA在感染后第 2天至第 16天均可测出 ,其中上清液中HBsAg、HDAg以感染后第 4天至第 12天达高峰。 结论 :HDV在原代培养人胎肝细胞中能稳定复制和表达至少达 12d。
Objective: To establish an in vitro culture system of HDV / HBV infected human fetal liver cells. Methods: HDV / HBV positive sera were used to infect human fetal hepatocytes cultured in vitro. HBsAg, HDAg, HBVDNA and HDV cDNA in supernatant and cells were detected by ELISA, immunohistochemistry, in situ hybridization and dot blotting. Results: HBsAg, HDAg, HBVDNA and HDV cDNA in supernatant and cells were detected on the 2nd day to the 16th day after infection, and the highest level of HBsAg and HDAg in the supernatant reached the peak on the 4th day to the 12th day after infection . Conclusion: HDV can stably replicate and express in primary cultured human fetal hepatocytes for at least 12 days.