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研究了在玻碳电极利用巯基乙胺固定纳米金、然后纳米金固载癌胚抗体(Ab1),采用脂质体包裹电子媒介体硫堇,脂质体周围联接标记辣根过氧化物酶(HRP)的癌胚抗体(Ab2)对其传感器进行信号放大,通过循环伏安法考察了该免疫传感器的电化学特性,在优化的实验条件下,该免疫传感器的峰电流随着检测溶液中癌胚抗原(CEA)浓度的增大而增大,并在0.05～200 ng/mL CEA范围内呈现线性关系,回归方程为:Δi=0.20+0.24ρ(ng/mL);检测限为:18pg/mL(R=0.9947)。该免疫传感器可用于临床上对CEA的检测。
Gold nanoparticles were immobilized on the glassy carbon electrode with mercaptoethylamine, and then immobilized with gold nanoparticle to immobilize the carcinoembryonic antigen (Ab1). The electron mediator thionine was encapsulated by liposomes and labeled with horseradish peroxidase HRP) of the carcinoembryonic antibody (Ab2) signal amplification of its sensor, the electrochemical properties of the immunosensor was investigated by cyclic voltammetry under optimal experimental conditions, the peak current of the immunosensor with the detection of cancer in solution (CEA), and showed a linear relationship in the range of 0.05 ~ 200 ng / mL CEA.The regression equation was: Δi = 0.20 + 0.24 ρ (ng / mL), and the detection limit was 18 pg / mL (R = 0.9947). The immunosensor can be used to detect CEA clinically.