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目的:通过检测HBeAg阴性慢性乙肝患者血清表面抗原大蛋白(HBV-LP)、PreS1蛋白、PreS2蛋白和HBV-DNA比较分析,以探讨血清乙肝大蛋白检测对于HBeAg阴性慢性乙肝患者在临床诊治中的价值和意义。方法:采用ELISA法检测247例HBeAg阴性慢性乙肝患者HBV-LP、PreS1蛋白、PreS2蛋白,并应用实时荧光定量PCR技术平行检测HBV-DNA。结果:247例HBeAg阴性慢性乙肝患者HBV-DNA阳性率51.0%,血清HBV-LP阳性率66.4%,PreS1蛋白阳性率22.3%,PreS2蛋白阳性率34.0%,血清中HBV-LP阳性率明显高于PreS1蛋白和PreS2蛋白阳性率,差异均有显著性(P均<0.01)。结论:HBeAg阴性慢性乙肝患者检测其血清HBV-LP较PreS1蛋白和PreS2蛋白更敏感反映体内HBV感染和复制状态,HBV-LP更有利于HBeAg阴性慢性乙肝患者的疗效观察和预后判断。
OBJECTIVE: To compare the serum level of surface antigen antigen protein (HBV-LP), PreS1 protein, PreS2 protein and HBV-DNA in patients with HBeAg-negative chronic hepatitis B in order to investigate the detection of serum hepatitis B protein in clinical diagnosis and treatment of HBeAg-negative chronic hepatitis B patients Value and meaning. Methods: HBV-LP, PreS1 protein and PreS2 protein were detected by ELISA in 247 patients with HBeAg-negative chronic hepatitis B. HBV-DNA was detected by real-time fluorescence quantitative PCR. Results: The positive rate of HBV-DNA in 247 HBeAg-negative chronic hepatitis B patients was 51.0%, the positive rate of serum HBV-LP was 66.4%, the positive rate of PreS1 protein was 22.3%, the positive rate of PreS2 protein was 34.0%, and the positive rate of HBV-LP in serum was significantly PreS1 protein and PreS2 protein positive rate, the difference was significant (P all <0.01). Conclusions: The detection of serum HBV-LP in HBeAg-negative chronic hepatitis B patients is more sensitive than PreS1 protein and PreS2 protein to reflect HBV infection and replication status in vivo. HBV-LP is more beneficial to the observation and prognosis of HBeAg-negative chronic hepatitis B patients.