在酶联免疫吸附试验 (ＥＬＩＳＡ)中固定半抗原通常采用与蛋白分子相偶联或共价结合到固相载体上。这样的步骤既费时也可能影响其抗原性 ,导致不适当的抗原呈逆和定位 ,这里介绍一种将低分子量半抗原直接结合到聚苯乙烯板上的方法 :以Ｐ 氨基 Ｄ 苯丙氨酸与固相支持物ＥＬＩＳ Immobilization of haptens in enzyme-linked immunosorbent assays (ELISA) is usually carried out by coupling to a protein molecule or by covalent attachment to a solid support. Such steps are both time-consuming and may affect their antigenicity, resulting in inappropriate antigen reversal and localization. Here is a description of a method of binding low molecular weight haptens directly to polystyrene plates: P-amino-D-phenylalanine With solid support ELIS