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人癌细胞体外培养是研究癌变机理和癌细胞生物学特性的重要方法。在肺癌细胞培养方面,1955年Frisch首先报告了肺腺癌患者胸水细胞建成的MABEN细胞系,1962年国内何申也报道了人肺鳞癌细胞系。随后,又有众多关于肺癌细胞建系的报道。本文就近年来人肺癌细胞体外培养技术的改进和应用研究两方面作一综述。一、人肺癌细胞体外培养(一)材料和方法肺部原发癌、转移癌,恶性渗液,淋巴结活检,骨髓穿刺及其它转移部位切取的癌组织标本均可用作体外培养的
The in vitro culture of human cancer cells is an important method for studying the mechanism of canceration and the biological characteristics of cancer cells. In lung cancer cell culture, in 1955 Frisch first reported the establishment of MABEN cell line in patients with lung adenocarcinoma pleural effusion cells. In 1962, He Shen also reported human lung squamous cell carcinoma cell line. Subsequently, there were many reports on the establishment of lung cancer cells. This article reviews the recent advances in the improvement and application of human lung cancer cell culture in vitro. I. In vitro culture of human lung cancer cells (I) Materials and methods Primary lung cancer, metastatic cancer, malignant effusion, lymph node biopsy, bone marrow biopsy, and other cancerous tissue specimens taken from other metastatic sites can be used for in vitro culture.