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为探讨青石棉与ｐ５３基因突变之间的关系，采用ＰＣＲ－ＳＳＣＰ分析方法，对８株青石棉诱发的ＢＡＬＢ／ｃ３Ｔ３转化细胞进行ｐ５３基因的突变检测和分析。结果：从８株转化细胞ｐ５３基因上的１１个外显子中共发现７处突变，其中２个位于外显子４－６，５个位于外显子９－１１；大多数检出的异常表现为比野生型多１条带；突变随机地分布于各个青石棉剂量组。由此表明，ｐ５３基因突变与体外转化实验中所用的青石棉剂量无关，检出的突变多位于外显子９－１１内，与人类肿瘤中常见ｐ５３基因突变多发生在外显子５－８的情形有所不同。文内还对青石棉与ｐ５３基因突变之间的关系进行了讨论
To investigate the relationship between crocidolite and p53 gene mutation, PCR-SSCP was used to detect and analyze the mutation of p53 gene in 8 crocidolite-induced BALB / c3T3 transformed cells. RESULTS: Seven mutations were found in 11 exons of p53 gene in 8 transformed cells, of which 2 were located in exon 4-6 and 5 were located in exon 9-11. Most of the abnormalities were detected One more band than the wild type; mutations were randomly distributed in each crocidolite dose group. Thus, p53 mutation was not related to the dose of crocidolite used in the in vitro transformation experiments. The mutations detected were mostly located in exon 9-11. Mutations of p53 gene commonly found in human tumors occurred in exons 5-8 The situation is different. The article also discussed the relationship between crocidolite and p53 gene mutations