目的探讨白纹伊蚊唾液腺细胞的培养,试图建立白纹伊蚊唾液腺细胞的原代和传代培养。方法采用Schneider培养基添加10%的胎牛血清和双抗﹙100U/ml青霉素,100μg/ml链霉素﹚作培养液,在无菌条件下取出唾液腺,经PBS液清洗后放入培养液中,置26℃培养箱中培养。结果3d后白纹伊蚊唾液腺组织开始长出单个细胞,6d后单个细胞增多。说明白纹伊蚊唾液腺细胞在体外培养条件下能够存活一段时间。结论白纹伊蚊唾液腺细胞体外培养存活。 Objective To investigate the culture of salivary gland cells of Aedes albopictus in order to establish the primary and subculture of salivary gland cells of Aedes albopictus. Methods Schneider medium supplemented with 10% fetal bovine serum and double antibody (100U / ml penicillin, 100μg / ml streptomycin) was used as the culture medium. The salivary glands were removed under aseptic conditions, washed in PBS and placed in culture medium , Set at 26 ℃ incubator culture. Results After 3d, salivary glands of Aedes albopictus began to grow single cells, and single cells increased after 6 days. It shows that Aedes albopictus salivary gland cells can survive for a period of time under in vitro culture conditions. Conclusion Aedes albopictus salivary gland cells survive in vitro.