论文部分内容阅读
Ti转化的丹参组培物在MS-NH4培养基(MS中不含硝酸铵,含蔗糖 30 g*L-1)上经25 ℃暗培养18 d,生长量由大到小依次为:对照(CK),200 μmol *L-1水杨酸(SA),4 g*L-1酵母激发子(E)和E+SA处理。其中CK和SA处理的组培物仅产生痕量的丹参酮类物质,而E和E+SA处理的总丹参酮产量分别增加到12.23 mg* L-1和15.07 mg*L-1,表明E处理能促进组培物产生丹参酮类物质,而SA可以强化E的这种作用。与上述差异有关的内源激素变化是:培养6~18 d,E+SA处理的ABA和i PAs含量均持续高于对照,分别增长了2.8~9.8倍和3.6~5.8倍,而GA1+3和IAA 含量分别比对照降低13.2%~56.9%和34.8%~74.6%。高水平的ABA和iPAs及低水平GA 1+3和IAA抑制了组培物的生长,有利于促进丹参酮产量的提高。“,”The fresh weight and dry weight of Ti-transformed Salvia miltiorrhiza cell aggregates increase differently with various treatm ents,after being cultured in the MS-NH4 medium(MS without ammonium nitrate,co ntaining 30 g*L-1 sucrose)under darkness for 18 days(25 ℃).The total tanshinones production reaches 12.23 mg*L-1 and 15.07 mg*L-1 by exogenous application of 4 g*L-1 of yeast elicitors(E)and E+200 μmol*L-1 of salicylic acid(SA)respectiv ely.Howeve r,only trace amount of tanshinones was detected in the control and SA treatment, indicating that E could promote tanshinones production and SA enhances the effect of E.Compared with the control,the contents of ABA and iPAs in E+SA treated cell aggregates rise continuously and with an increase of 2.8-9.8 and 3.6-5. 8 fold respectively,but those of GA1+3 and IAA are decreased by 13.2%-56 .9% and 34.8%-74.6% respectively,suggesting that high level of ABA and iPAs combined with low level of GA1+3 and IAA inhibits the growth of cell aggre gates,and therefore significantly stimulates the tanshinones production.