实验采用雄性大鼠20只,随机分为实验组和对照组。实验组用杀虫畏(150mg/kg)溶于麻油灌胃,1次/d 连续7d,对照组给等量麻油。末次给药后24h,取肝脏制备微粒体,测定苯胺羟化酶活力。另外分别制备两组动物的肝微粒体混合样品,测定在不同底物浓度时(5×10~(-5)～1×10~(-4)mol/L)两组苯胺羟化酶活力,计算米氏常数(Km)。同时另取微粒体混合样品,分别加入微粒体酶抑制剂 Twenty male rats were randomly divided into experimental group and control group. Experimental group with dicamba (150mg / kg) dissolved in sesame oil, 1 / d for 7 days, the control group to equal sesame oil. 24h after the last administration, microsomes were prepared from the liver and the aniline hydroxylase activity was measured. In addition, the liver microsome samples of two groups of animals were separately prepared to measure the aniline hydroxylase activities of the two groups at different substrate concentrations (5 × 10 -5 to 1 × 10 -4 mol / L) Calculate the Michaelis constant (Km). At the same time another microsomal mixed samples were added microsomal enzyme inhibitors