将表达Ro60kDGST融合蛋白的重组体导入大肠杆菌JM109 中表达重组抗原, 经GST亲和层析柱纯化后用标准抗Ro血清鉴定, 经免疫印迹法证实, 纯化重组蛋白具有Ro 抗原性, 该抗原可用于临床检测及研究抗原表位与疾病的相关性 The recombinant protein expressing Ro60kDGST fusion protein was introduced into E.coli JM109 to express the recombinant antigen. After purified by GST affinity chromatography, the recombinant protein was identified by standard anti-Ro serum. The purified recombinant protein had Ro antigenicity by immunoblotting. Antigens can be used in clinical testing and research antigen epitopes and disease related