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为探索郁金香的组织培养方法,本试验以叶片、花茎、花托、鳞片、芽为外植体,研究不同外植体对郁金香组织培养中愈伤诱导的影响。结果表明:内层鳞片、花托和花茎接种一段时间后只有愈伤萌动迹象,但萌动后,则不再分化,逐渐褐化死亡;叶片接种后,无愈伤分化萌动,直接褐化死亡;2℃和5℃处理12周后鳞茎的芽的诱导率和鳞片的萌动率较高;鳞茎2℃处理12周后的芽接种在培养基MS﹢NAA0.3 mg/L﹢6-BA 2.0 mg/L上,其愈伤组织诱导率可以达到50%。所以,在本实验中,以2℃处理12周后鳞茎的芽作外植体诱导愈伤的效果最好,本实验中培养基MS﹢NAA0.3 mg/L﹢6-BA 2.0 mg/L适宜芽诱导愈伤。
In order to explore the methods of tissue culture of tulip, the effects of different explants on the callus induction in tissue culture of tulip were studied in this experiment with explants of explants such as leaves, flower stems, torus, scales and buds. The results showed that only the signs of callus germinated after inoculation of inner scales, receptacle and flower stems for a period of time, but no differentiation and gradual browning died after inoculation; without callus differentiation and direct browning after inoculation; 2 After 12 weeks of treatment at 2 ℃, the buds of bulbs were inoculated with MS + NAA 0.3 mg / L and 6-BA 2.0 mg / L, the callus induction rate can reach 50%. Therefore, in this experiment, shoots of bulblets were the best explants for callus induction at 12 ℃ for 2 weeks. MS + NAA 0.3 mg / L + 6-BA 2.0 mg / L Appropriate bud-induced callus.