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目的 :通过体外分离、增殖培养获取γδT细胞、NK细胞和LAK细胞 ,并比较了 3种细胞的抗肿瘤的生物学特性。方法 :收集用不同单抗分别包装被粘附的细胞 ,然后通过MACS细胞分选仪的分选 ,获取的细胞进行细胞增殖动力学、细胞表型、细胞杀伤活性的测定以单抗阻断效应的分析。结果 :经MACS分离得到的γδT细胞 ,培养 2w后细胞数扩散 6 0 0～ 80 0倍 ,CD3、CD8和γδ细胞表达阳性率分别是 72 2 9%、5 8 0 2 %和 6 5 98%。γδT细胞对NK敏感细胞K5 6 2以及NK不敏感细胞Raji和XG 7这 3种不同靶细胞均有较高的杀伤率 ,分别为 35 98%、42 2 7%和 6 9 0 8% ;NK细胞对此 3种细胞的杀伤率分别是45 12 %、12 34%和 2 9 2 7% ;LAK细胞的杀伤率分别为 44 0 1%、2 9 2 7%和 2 5 6 8%。γδT细胞对经MHC Ⅰ类单抗阻断前后的K5 6 2、Raji和XG 73种靶细胞的杀伤率无明显改变。结论 :γδT细胞、NK细胞和LAK细胞都具有一定的非特异性杀伤肿瘤细胞的作用 ;γδT细胞对MHC Ⅰ类单抗阻断后的肿瘤细胞的杀伤无明显变化 ,提示γδT细胞较NK细胞和LAK细胞有更广泛的抗瘤谱
Objective: To obtain γδT cells, NK cells and LAK cells by in vitro isolation and proliferation culture, and to compare the anti-tumor biological characteristics of the three types of cells. METHODS: The adhered cells were collected and packaged with different monoclonal antibodies. The cell proliferation kinetics, cell phenotype, and cell killing activity of the cells obtained by sorting using the MACS cell sorter were determined by the monoclonal antibody blocking effect. Analysis. RESULTS: After γδT cells isolated from MACS, the number of cells diffused 200-80 times after 2 weeks of culture, and the positive rates of CD3, CD8 and γδ cells were 72 2 9%, 580 2%, and 6 5 98%, respectively. . γδT cells had higher killing rates for NK sensitive cell K562 and NK insensitive cells Raji and XG7, which were 35 98%, 42 2 7% and 6 9 0 8%, respectively. NK The cytotoxicity rates of these three types of cells were 45 12%, 1234%, and 2937%, respectively; the LAK cell killing rates were 44 0 1%, 2 9 2 7%, and 2 5 6 8%, respectively. The γδT cells showed no significant changes in killing rates of K562, Raji, and XG 73 target cells before and after blocking by MHC class I monoclonal antibodies. CONCLUSION: γδT cells, NK cells and LAK cells all have certain effects on killing tumor cells non-specifically; γδT cells have no obvious changes in the killing of tumor cells after MHC class I monoclonal antibody blocking, suggesting that γδT cells are more effective than NK cells and LAK. Cells have a broader spectrum of anti-tumor