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目的 从超微水平了解P gp、p5 3蛋白、Bcl 2蛋白在肺癌组织中的表达 ,探讨它们与多药耐药 (multidrugresistance ,MDR)的关系和可能的作用机制。 方法 8例非小细胞肺癌 (NSCLC)手术标本和 7例小细胞肺癌 (SCLC)经支气管镜活检标本 ,用免疫电镜方法包埋后 ,以PAG标记抗体方法检测P gp、p5 3蛋白、Bcl 2蛋白的表达。 结果 P gp主要位于胞膜和胞浆内质网附近 ;p5 3主要位于细胞核异染色质上 ,胞浆中也有存在 ;Bcl 2分布与线粒体和内质网有关。三者在 15例肺癌中的表达率分别为 33 3% (5 / 15 )、6 0 0 % (9/ 15 )和 2 6 7% (4 / 15 )。 8例手术获得的正常肺组织标本中 ,3种蛋白表达均为阴性。 5例P gp阳性的标本中 ,4例为NSCLC ,仅 1例为化疗后的SCLC。 结论 用免疫电镜方法检测 ,P gp、p5 3蛋白、Bcl 2蛋白在肺癌中有一定的表达。P gp与p5 3、p5 3与Bcl 2的表达无相关性。P gp主要位于胞膜 ,支持其膜上药物输出泵的功能 ,其表达在肺癌的MDR中起一定作用 ,但不是惟一因素。
Objective To understand the expression of P gp, p53 protein and Bcl 2 protein in lung cancer tissues at ultra-micro level and to explore their relationship with multidrug resistance (MDR) and possible mechanisms. Methods 8 cases of non-small cell lung cancer (NSCLC) and 7 cases of small cell lung cancer (SCLC) were examined by bronchoscopic biopsy specimens and embedded by immunoelectron microscopy. Pgp, p53 protein and Bcl 2 were detected by PAG-labeled antibody method. Protein expression. Results The P gp was mainly located in the plasma membrane and cytoplasmic endoplasmic reticulum. p53 was mainly located in the cytoplasm of heterochromatin and cytoplasm. Bcl 2 distribution was related to mitochondria and endoplasmic reticulum. The expression rates of the three in lung cancer were 33 3% (5 / 15), 60% (9 / 15) and 267% (4 / 15). Eight of the normal lung tissue samples obtained by surgery were negative for the expression of all three proteins. Of the 5 P-gp-positive specimens, 4 were NSCLC and only 1 was SCLC after chemotherapy. Conclusion The expression of P gp, p53 protein and Bcl 2 protein in lung cancer was detected by immunoelectron microscopy. There was no correlation between P gp and p53, p53 and Bcl2 expression. Pgp is mainly located in the membrane and supports the function of the drug output pump on the membrane. Its expression plays a role in the MDR of lung cancer, but it is not the only factor.