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目的:观察不同孔径的多孔β-TCP分别复合庆大霉素及庆大霉素/壳聚糖后在体外庆大霉素的释放情况,研究多孔β-TCP孔径及壳聚糖对庆大霉素释放的影响。方法:利用冷冻干燥法使不同孔径的多孔β-TCP分别复合庆大霉素及庆大霉素/壳聚糖。比色法测定样本液内硫酸庆大霉素浓度,抑菌环实验测定样本液中庆大霉素的抗菌性。结果:(1)多孔β-TCP/庆大霉素中庆大霉素的释放时间分别为72h(187-300μm)、48h(300-375μm)、48h(375-500μm)、48h(500-750μm)、48h(750-830μm),孔径187-300μm组释放时间最长。(2)多孔β-TCP/庆大霉素/壳聚糖样本中庆大霉素的释放时间分别为12d(187-300μm)、12d(300-375μm)、12d(375-500μm)、13d(500-750μm)、12d(750-830μm),较β-TCP/庆大霉素中时间长,且在500-750μm上作用最显著。结论:(1)孔径对于庆大霉素释放具有一定的影响作用,187-300μm是延长多孔β-TCP/庆大霉素释放的最适孔径(在187-830μm内),但是时间太短。(2)壳聚糖能够显著延长庆大霉素在β-TCP上的释放时间,而在500-750μm孔径上作用最强。
OBJECTIVE: To observe the release of gentamicin in vitro by porous β-TCP with different pore sizes and with gentamicin and gentamycin / chitosan respectively. The effects of porous β-TCP pore size and chitosan on gentamicin The impact of prime release. Methods: The porous β-TCP with different pore sizes were respectively combined with gentamicin and gentamicin / chitosan by freeze-drying method. The colorimetric method was used to determine the concentration of gentamicin sulfate in the sample solution and the antibacterial ring test was used to determine the antibacterial activity of gentamicin in the sample solution. Results: (1) The release time of gentamicin in porous β-TCP / gentamicin was 72h (187-300μm), 48h (300-375μm), 48h (375-500μm), 48h ), 48h (750-830μm), pore size of 187-300μm group longest release time. (2) The release time of gentamicin in the porous β-TCP / gentamicin / chitosan samples was 12d (300-375μm), 12d (375-500μm), 13d 500-750μm), 12d (750-830μm), which was longer than that of β-TCP / gentamicin, and the most obvious effect was at 500-750μm. Conclusions: (1) The pore size has an effect on the release of gentamycin. The optimum pore size (within 187-830μm) is 187-300μm, but the time is too short to prolong the release of porous β-TCP / gentamicin. (2) Chitosan can significantly prolong the release time of gentamicin on β-TCP, while it has the strongest effect on the pore size of 500-750 μm.