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目的研究脑缺血及脑缺血再灌流大鼠脑内Ｐ物质（ＳＰ）、神经激肽Ａ（ＮＫＡ）、降钙素基因相关肽（ＣＧＲＰ）、生长抑素（ＳＳ）含量变化及其意义，为临床探讨脑缺血的病理生理过程及指导治疗提供科学依据。方法应用四血管关闭全脑缺血模型及再灌流模型，借助放射免疫分析法，对大鼠全脑缺血５，１０，１５，２０，３０，６０，１２０ｍｉｎ７个时点４个脑区（皮质、纹状体、下丘脑、中脑）和垂体的ＳＰ、ＮＫＡ、ＣＧＲＰ、ＳＳ含量变化进行观察，在此基础上测定缺血５ｍｉｎ再灌流５ｍｉｎ、缺血１５ｍｉｎ再灌流４５ｍｉｎ、缺血３０ｍｉｎ再灌流３０ｍｉｎ各脑区及垂体四种神经肽的变化。结果大鼠脑缺血后脑区、垂体ＳＰ、ＮＫＡ、ＣＧＲＰ、ＳＳ含量的变化规律不尽相同，但大部分呈现下降趋势。缺血５ｍｉｎ尚处于可逆状态，缺血５ｍｉｎ再灌流肽类呈不同程度的上升，缺血３０ｍｉｎ再灌流肽类上升不明显，甚至下降。缺血１５ｍｉｎ再灌流肽类变化介于上述两种情况之间。结论ＳＰ、ＣＧＲＰ、ＳＳ均为与学习、记忆过程有关的神经肽，在脑缺血后其含量的降低可能与脑缺血后智能障碍有关。从实验动物的神经肽角度看，应在缺血超早期（＜１５ｍｉｎ）积极恢复血液供应。ＣＧＲＰ对缺血性脑损伤是一?
Objective To investigate the changes of substance P (SP), neurokinin A (NKA), calcitonin gene related peptide (CGRP) and somatostatin (SS) in brain of rats with cerebral ischemia and reperfusion , To provide a scientific basis for clinical pathological and physiological process of cerebral ischemia and guiding treatment. Methods The model of global cerebral ischemia was established by four-vessel occlusion and the model of reperfusion was established. By radioimmunoassay, , Striatum, hypothalamus, midbrain) and pituitary gland SP, NKA, CGRP, SS content changes observed on the basis of this determination of ischemia 5min reperfusion 5min, ischemia 15min reperfusion 45min, ischemia 30min reperfusion 30min brain and pituitary changes of the four neuropeptides. Results The changes of contents of SP, NKA, CGRP and SS in brain area and pituitary were different after cerebral ischemia, but most of them showed a decreasing trend. Ischemia 5min is still in a reversible state, ischemic 5min reperfusion peptide increased to varying degrees, 30min ischemia reperfusion peptide rise was not obvious, or even decreased. Ischemia 15min reperfusion peptide changes between the above two cases. Conclusion SP, CGRP and SS are all neuropeptides related to learning and memory. The decreased content of neuropeptides after cerebral ischemia may be related to post-ischemic mental retardation. From the perspective of neuropeptides in experimental animals, the blood supply should be actively restored in the early stage of ischemia (<15 min). Is CGRP an ischemic brain injury?