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目的:建立以高效液相色谱法同时测定羚羊清肺散中绿原酸、栀子苷、芍药苷和甘草苷的含量的方法。方法:采用双波长HPLC法,Kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸水梯度洗脱,流速1.0 m L·min-1,检测波长327 nm(绿原酸),237 nm(栀子苷、芍药苷、甘草苷)。结果:绿原酸、栀子苷、芍药苷和甘草苷4种成分进样量分别在1.579~78.96μg(r=0.999 8),1.261~63.04μg(r=1),0.364~18.2μg(r=0.999 9),0.329 6~16.48μg(r=0.999 9)与峰面积线性关系良好;平均回收率分别为97.6%,97.6%,97.1%和99.8%,RSD分别为1.0%,0.7%,1.2%和1.3%。结论:该方法简便、快速、准确,可用于羚羊清肺散的质量控制。
Objective: To establish a method for simultaneous determination of chlorogenic acid, geniposide, paeoniflorin and glycyrrhizin in Antelope Qingfei San by high performance liquid chromatography. Methods: A two-wavelength HPLC method was used on a Kromasil C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase was eluted with a gradient of acetonitrile-0.1% phosphoric acid. The flow rate was 1.0 m L · min- Acid), 237 nm (geniposide, paeoniflorin, glycyrrhizin). Results: The contents of chlorogenic acid, geniposide, paeoniflorin and liquiritin were 1.579 ~ 78.96μg (r = 0.999 8), 1.261 ~ 63.04μg (r = 1) and 0.364 ~ 18.2μg = 0.999 9). The linear range of 0.329 6 ~ 16.48μg (r = 0.999 9) was linear with the peak area. The average recoveries were 97.6%, 97.6%, 97.1% and 99.8% respectively. The RSDs were 1.0%, 0.7%, 1.2 % And 1.3%. Conclusion: The method is simple, rapid and accurate and can be used for quality control of Antelope Qingfei San.