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目的探讨丙型副伤寒、猪霍乱以及汤卜逊沙门菌的血清型鉴定要点,评价不同诊断血清的鉴定能力。方法用三家不同生产厂家的沙门菌诊断血清对丙型副伤寒、猪霍乱、猪霍乱库氏变种沙门菌三株标准株以及GSS中国区监测的疑似丙型副伤寒暴发分离株、猪霍乱和汤卜逊沙门菌进行血清鉴定,同时利用PCR、生化、以及MLST分型分析对这些菌株进行辅助分析。结果血清分型显示暴发菌株为汤卜逊沙门菌,那些原来鉴定为猪霍乱沙门菌的菌株也是汤卜逊沙门菌;PCR结果显示这些暴发菌株和散发菌株的Vi基因为阴性;还发现某些诊断血清的H:c因子血清能够与H:k抗原发生交叉凝集,从而导致血清型的误判;另外,MLST分型分析显示这些汤卜逊沙门菌菌株与丙型副伤寒、猪霍乱等沙门菌之间有差异;卫矛醇、粘液酸和H2S三种生化反应显示丙型副伤寒、猪霍乱、猪霍乱库氏变种沙门菌之间具有明显的生化差别。结论近期我国报告为丙型副伤寒的暴发中,需要鉴定是否为汤卜逊沙门菌所致,注意诊断血清的交叉反应所致的误判,可用生化鉴定、MLST分型分析以及PCR等方法进一步鉴定。
Objective To investigate the serotypes of Salmonella paratyphi A, cholera and Typhimurium thompson and to evaluate the diagnostic ability of different diagnostic sera. Methods Three Salmonella serogroups from three different manufacturers were used to diagnose Seroprevalence of Salmonella paratyphi A, Cholera serogroups, three strains of Salmonella choleraesuis mutants and three strains of suspected Salmonella paratyphoid isolates that were monitored by GSS China, Salmonellapulsi were used for serological identification, and PCR, biochemical and MLST genotyping analyzes were performed for these strains. Results The serotyping showed that the outbreak strain was Salmonella Thompson, and those strains originally identified as Salmonella choleraesuis were also Salmonella Thompson; PCR results showed that the Vi genes of these outbreaks and strains were negative; some Serum H: c factor serum can cross-aggregate with H: k antigen, resulting in misjudgment of serotypes. In addition, MLST typing analysis showed that these Salmonella typhimurium strains were associated with Salmonella paratyphoid and swine cholera and other Salmonella There are differences between the bacteria; daccosyl alcohol, mucic acid and H2S three biochemical reactions showed Paratyphoid C, cholera, pig cholera Cumulas Salmonella has obvious biochemical differences. Conclusion In the recent report of China as an outbreak of paratyphoid A, it is necessary to identify whether it is caused by Salmonella Thompson. The misjudgment caused by the diagnosis of serum cross-reactivity can be confirmed by biochemical identification, MLST typing and PCR Identification.