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目的观察脊髓损伤(SCI)后补阳还五汤是否有促进移植间充质干细胞(MSC)转分化为神经元作用。方法SD大鼠30只,以自制改良Allen装置造模成功后,随机分为模型组(不治疗)、补阳还五汤组、补阳还五汤加MSC组各10只。补阳还五汤组大鼠造模后当天即开始每天一次中药灌胃(3g/kg);补阳还五汤加MSCs组大鼠造模后随即移植MSC,细胞浓度1×106/mL,每鼠4.5!L,注入大鼠蛛网膜下隙,注射速度1!L/min,并开始每天一次中药灌胃,剂量3g/kg。4周后观察脊髓结构变化。结果与模型组相比,补阳还五汤组脊髓组织结构明显改善,前角运动神经元数量有所增加;补阳还五汤加MSC组结构最佳,并且前角运动神经元数量最多。结论由此推测补阳还五汤可能有促进移植MSC转分化为脊髓前角运动神经元作用。
Objective To observe whether Buyang Huanwu Decoction can promote the transdifferentiation of transplanted mesenchymal stem cells (MSCs) into neurons after spinal cord injury (SCI). Methods Thirty SD rats were successfully divided into model group (untreated), Buyang Huanwu Decoction group and Buyang Huanwu Decoction plus MSC group. The rats in Buyang Huanwu Decoction group were given intragastric administration once a day (3g/kg) on the day after the model was established; the rats in Buyang Huanwu Decoction plus MSCs group were transplanted with MSC immediately after the model was established, and the cell concentration was 1×10 6 /mL. Rats 4.5!L were injected into the subarachnoid space of rats at an injection rate of 1 L/min, and once daily, the rats were intragastrically administered at a dose of 3 g/kg. After 4 weeks, the structural changes of the spinal cord were observed. Results Compared with the model group, the spinal cord structure of Buyang Huanwu Decoction group was significantly improved, and the number of motoneurons in the anterior horn increased; the Buyang Huanwu Decoction plus MSC group had the best structure, and the number of anterior horn motor neurons was the highest. Conclusion It is speculated that Buyang Huanwu Decoction may promote the transdifferentiation of transplanted MSCs into spinal motoneurons.