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双子叶植物的下胚轴和单子叶植物的中胚轴的生长模式是暗形态建成和光形态建成的重要标志性发育事件,黑暗促进其延伸,而光照则抑制其生长。拟南芥下胚轴的形态建成受光信号通路与植物激素途径中许多重要基因的调控,这些基因在农作物中的同源基因,往往也与许多重要的农艺性状有关。基于此,以玉米的中胚轴在暗形态建成中的长度作为表型标记,从EMS突变体库中(包含6 150份郑58背景株系和2 340份B73背景株系)筛选获得96株中胚轴长度异常的突变体。进一步对其中两个突变体进行了初步分析,发现这两个突变体在BR信号途径发生缺陷。玉米暗形态建成突变体的获得将不仅有助于解析玉米对光信号的应答,而且有助于揭示光信号通路与其他信号途径的互作。
The hypocotyls of dicotyledonous plants and mesocotyls of monocotyledonous plants are important landmark events in dark morphogenesis and light morphogenesis. Darkness promotes its extension, while light inhibits its growth. The morphology of Arabidopsis hypocotyls is regulated by many important genes involved in signal pathways and plant hormone pathways. The homologous genes of these genes in crops are often related to many important agronomic traits. Based on this, taking the length of the mesodermal axis of maize in the dark morphogenesis as a phenotypic marker, 96 strains were screened from the EMS mutant library (including 6 150 Zheng 58 background lines and 2 340 B73 background lines) Hypocotyl length mutant. Further analysis of two of the two mutants revealed that these two mutants had defects in the BR signaling pathway. Acquisition of maize dark morphologically constructed mutants will not only help to resolve maize response to light signals, but also help reveal the interaction of light signaling pathways with other signaling pathways.