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目的通过检测新生儿脐带血中人巨细胞病毒(human cytomegalovirus,HCMV)IgG抗体和gB基因,了解新生儿HCMV感染情况,并结合临床资料分析产妇年龄、Ⅰ型单纯疱疹病毒(herpes simplex virus,HSV-Ⅰ)IgG抗体阳性、孕周与HCMV先天性感染的相关性。方法收集2010年11月～2011年6月期间,某市妇幼保健院就诊的健康产妇所娩新生儿脐带血,共178例。采用ELISA法检测脐带血血清是否存在HCMV IgG和HSV-ⅠIgG抗体,巢式PCR检测HCMV gB基因。结果产妇HCMV IgG阳性率为99.44%(177/178);产妇HSV-ⅠIgG阳性率为83.71%(149/178);新生儿脐血HCMV gB阳性率为32.02%(57/178);产妇年龄和HSV-ⅠIgG抗体阳性不是发生HCMV先天性传播的危险因素。结论针对早产和过期产儿,应该加强其HCMV先天性感染的早期诊断。
OBJECTIVE: To detect HCMV infection and gB gene in neonatal umbilical cord blood to understand the status of HCMV infection in newborns. Combined with clinical data, maternal age, herpes simplex virus (HSV) -I) IgG antibody-positive, gestational age and HCMV congenital infection. Methods From November 2010 to June 2011, a total of 178 newborn infants born from healthy mothers in a city MCH hospital were collected. HCMV IgG and HSV-Ⅰ IgG were detected by ELISA in cord blood serum, and gB gene was detected by nested PCR. Results The positive rate of HCMV IgG in pregnant women was 99.44% (177/178). The positive rate of HSV-Ⅰ IgG in pregnant women was 83.71% (149/178). The positive rate of HCMV gB in neonates was 32.02% (57/178) HSV-ⅠIgG antibody is not the risk of congenital transmission of HCMV risk factors. Conclusion For preterm and postpartum infants, early diagnosis of congenital HCMV infection should be strengthened.