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目的 观察碘、氟对大鼠子代脑发育形态学方面是否存在联合作用。方法 将实验用Wistar成年大鼠雌 1 6只 ,雄 8只 ,按饮水中不同碘、氟或碘 +氟浓度分为 8组 ,即 :碘、氟为 0 (对照组 ,采用重蒸水 ) ,碘 0 5、 1 0、 2 0mg/L ,氟 50mg/L ,碘 +氟 0 5+ 50、 1 0 + 50、 2 0 + 50mg/L。喂养 90d后相同浓度组雌、雄按 2∶1合笼交配 ,交配后 2 1～ 2 8d母鼠产仔 ,哺乳 5d后将各组仔鼠断头处死。取其脑组织进行大脑重量、光镜 (观察大脑皮质神经细胞密度及未分化神经母细胞占细胞总数百分比 )、电镜 (观察神经元发育情况 )等形态学指标检查。结果 仔鼠大脑重量及光镜指标经 2× 4析因方差分析均表明各组之间差异有显著性 (P <0 0 0 1 )。其中氟 50mg/L、碘 2 0mg/L及碘 +氟 2 0+ 50mg/L组各项指标都提示仔鼠大脑发育状况次于其余各组。电镜检查发现氟 50mg/L及碘 +氟 2 0 + 50ml/L组神经元发育及超微结构形态有轻微变性改变。结论 长期饲以高氟及高碘饮水的大鼠均可影响其子代大脑发育 ,且高碘、高氟对仔鼠大脑发育的危害存在协同作用
Objective To observe whether iodine and fluorine have a combined effect on the morphological development of rat offspring. Methods Sixteen female and eight male Wistar rats were divided into 8 groups according to different iodine, fluorine or iodine + fluoride concentrations in drinking water: iodine and fluoride (control group: distilled water) , Iodine 0 5, 10, 20 mg / L, fluorine 50 mg / L, iodine + fluorine 0 5+ 50, 10 + 50, 20 + 50 mg / L. After feeding for 90 days, the female and male in the same concentration group were mated by 2: 1 cage, and the females were sacrificed 21 to 28 days after mating. After 5 days of lactation, the females were decapitated. Take the brain tissue for brain weight, light microscopy (observed cortical nerve cell density and the percentage of undifferentiated neuroblasts accounted for the total number of cells), electron microscopy (observation of neuronal development) and other morphological indicators. Results The pups’ brain weight and light microscope index were analyzed by 2 × 4 factorial analysis of variance, which showed that there was significant difference among the groups (P <0.01). Fluorine 50mg / L, iodine 20mg / L and iodine + fluorine 20 + 50mg / L group indicators suggest that the offspring brain development status is inferior to the other groups. Electron microscopy revealed slight degeneration of neuronal development and ultrastructural changes in the groups of 50 mg / L fluoride and 20 +50 ml / L fluoride. Conclusion Long-term high fluoride and high iodine drinking rats can affect their offspring brain development, and high iodine, high fluoride on the development of offspring brain synergies