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目的:建立并评价异常黑胆质证哮喘病证结合模型。方法:采用多因素复合作用建立异常黑胆质证模型,结合卵蛋白致敏激发,进一步建立异常黑胆质证哮喘病证结合模型,观察其一般状态、行为学、下丘脑-垂体-肾上腺轴(HPAA)功能、交感/副交感神经功能、气道反应性、炎症、胶原沉积等相关指标的变化。结果:异常黑胆质证组、异常黑胆质证哮喘组出现体质量下降(P<0.05),饮食水量增加(P<0.05),在旷场试验中自主活动减少(P<0.05),对糖水偏爱程度下降(P<0.05)等表现,血浆促肾上腺皮质激素(ACTH)、皮质酮(CORT)水平升高(P<0.05),环磷酸腺苷(cAMP)、cAMP/环磷酸鸟苷(cGMP)下降(P<0.05);哮喘组、异常黑胆质证哮喘组出现气道阻力升高(P<0.05),血清、肺泡灌洗液中Th1/Th2细胞因子失衡,呈Th2优势型(P<0.05),HE染色显示炎细胞浸润明显(P<0.05),PAS染色提示黏液分泌增多(P<0.05),MASSON染色提示胶原沉积增多(P<0.05),其中,以异常黑胆质证哮喘组改变更为突出。结论:采用多因素复合和卵蛋白致敏激发建立的异常黑胆质证哮喘模型一方面具有类似维吾尔医异常黑胆质证的临床表现及相关生理学指标的改变,另一方面具有气道高反应性、气道炎症、气道重塑等哮喘疾病特征,表明该模型的建立具有一定的可行性。
Objective: To establish and evaluate the combined model of asthenospermia with abnormal savda syndrome. Methods: Abnormal savda syndrome model was established by multifactorial combination therapy. Combined with the ovalbumin sensitization, a combined model of abnormal savda syndrome was established. The general state, behavior, hypothalamic-pituitary-adrenal axis (HPAA) ) Function, sympathetic / parasympathetic function, airway responsiveness, inflammation, collagen deposition and other related changes. Results: Abnormal savda syndrome group and abnormal savda syndrome asthma group showed decreased body weight (P <0.05), increased dietary water intake (P <0.05), decreased voluntary activity in the open field test (P <0.05) (P <0.05). The levels of plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT) were significantly increased (P <0.05). The levels of cAMP, cAMP / cGMP, (P <0.05). Th1 / Th2 cytokines in serum and bronchoalveolar lavage fluid were unbalanced (P <0.05) in asthmatic group and asthmatic group with abnormal savda syndrome (P <0.05) (P <0.05); PAS staining showed increased mucus secretion (P <0.05); MASSON staining showed an increase of collagen deposition (P <0.05), among them, asthma group with abnormal savda syndrome changed more To highlight. Conclusion: The asthenia model of abnormal savda syndrome established by multifactorial recombination and ovalbumin challenge has the clinical manifestations and related physiological changes similar to Uyghur’s abnormal savda syndrome. On the other hand, it has the characteristics of airway hyperresponsiveness, Airway inflammation, airway remodeling and other asthma disease characteristics, indicating that the establishment of the model has a certain feasibility.