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目的分析云南省2003年急性弛缓性麻痹(acute flaccid paralysis,AFP)病例中非脊灰病毒(non-polio,NPV)的带毒状况和埃柯病毒7型(echovirus 7,E7)的基因特征。方法按Obster等介绍的方法,对2003年云南省脊灰实验室分离到的18株NPV进行基因测序定型并对埃柯7病毒的基因特征进行分析。结果 2003年共采集到<15岁AFP病例粪便标本215份,在34株阳性标本(带毒率为15.8%)中,脊髓灰质炎病毒(poliovirus,PV)16株(阳性率4.7%),均为疫苗株,未发现脊灰野病毒;非脊灰病毒(NPV)18株(阳性率8.4%),其中1株为人类肠道病毒A组(human enterovirus A,HEV-A,1个血清型),14株为人类肠道病毒B组(HEV-B,9个血清型,其中3株为埃柯病毒7型,echovirus7,E7),3株为人类肠道病毒C组(HEV-C,2个血清型),未分离到HEV-D组病毒。结论 2003年云南省AFP病例中非脊灰病毒携带率(8.4%)与往年持平,3株E7病毒存在基因多样性特点(即存在不同的基因型)。
Objective To analyze the virulence of non-polio (NPV) and the genetic characteristics of echovirus 7 (E7) in Yunnan Province in 2003 with acute flaccid paralysis (AFP). Methods According to the method introduced by Obster et al., 18 strains of NPV isolated from the Yunnan Provincial Polio Laboratory were genotyped and their genetic characteristics were analyzed. Results A total of 215 stool samples from 15-year-old AFP patients were collected in 2003. Out of 34 positive samples (15.8%), 16 were positive for poliovirus (positive rate 4.7%) 18 strains of non-poliovirus (NPV) (positive rate was 8.4%), of which 1 was human enterovirus A (HEV-A, 1 serotype) , 14 strains were human enterovirus group B (HEV-B, 9 serotypes, of which 3 were echovirus7 and e7) and 3 were human enterovirus C (HEV-C, 2 Serotype), did not isolate the HEV-D group of viruses. Conclusion The non-poliovirus carrying rate (8.4%) in AFP cases in Yunnan Province in 2003 was the same as in previous years. The genetic diversity of three E7 viruses (ie, different genotypes) existed.