论文部分内容阅读
用α 3 2 P dCTP逆转录分别标记成人正常鼻咽和鼻咽癌组织总RNA 5 μg ,将合成的cDNA探针与具有 5 184个点阵的高密度cDNA微阵列杂交 ,用软件分析两者表达谱差异结果发现 ,鼻咽癌组织中 ,密度值在 2 0 0以上有 187个EST ,鼻咽组织中 30 7个EST ,而 38个EST在鼻咽组织高表达但在鼻咽癌组织低表达 ,48个EST在鼻咽癌组织高表达但正常鼻咽组织低表达 .结果说明 ,正常鼻咽与鼻咽癌组织可能存在多个差异表达的新基因而在该瘤的发生中起作用 ;采用高密度cDNA微阵列是一种可在大范围内筛选差异表达基因的强有力方法
The total RNA of normal human nasopharyngeal and nasopharyngeal carcinoma tissue was labeled with α 3 2 P dCTP reverse transcription by 5 μg respectively. The synthesized cDNA probes were hybridized to a high density cDNA microarray with 5 184 dot matrixes and analyzed by software The difference in expression profile of nasopharyngeal carcinoma showed that there were 187 ESTs above 200 and nascent nasopharyngeal tissues, while 38 ESTs were highly expressed in nasopharyngeal tissues but low in NPC tissues 48 ESTs were highly expressed in nasopharyngeal carcinoma tissues but low in normal nasopharyngeal tissues.The results showed that there may exist many new differentially expressed genes in normal nasopharyngeal and nasopharyngeal carcinoma tissues and play a role in the occurrence of this tumor. The use of high-density cDNA microarrays is a powerful method for screening differentially expressed genes on a large scale