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疼痛感受的生理学研究表明 ,伤害性信息上传时在脊髓水平受到脊髓自身和上位脑结构的调控。大量研究还表明 ,在伤害性信息传入背角时 ,会引起即早基因 (immediate earlygenes)的大量表达 ;c fos基因作为即早基因中的一种 ,所产生的fos蛋白在痛调制中起重要作用。强啡肽A (dynorphinA ,DynA)主要存在于背角中间神经元 ,且主要集中于背角浅层 ;在慢性炎症性疼痛时 ,脊髓中强啡肽的含量大量增加 ,说明强啡肽在脊髓水平的痛信息处理中起重要作用 ,可能与脊髓痛感受的可塑性变化有关。本研究的目的是用反义寡聚核苷酸 (antisenseoligodeoxynucleotide ,AS ODN)技术 ,封闭背角中fos蛋白的表达生成 ,以观察Fos蛋白和慢痛反应的关系 ,并分析fos蛋白生成增加和DynA表达之间的关系 ,并进一步探讨强啡肽A在痛调制中的作用。实验在Wistar大鼠 (10 0～ 12 0 g)进行。作为预实验的空白对照组动物 (n =5 ) ,在一侧后肢脚掌皮下注射formalin (5 % ,5 0 μl)后 ,用计算动物舔咬注射侧后脚掌的累计时间的方法 ,证明大鼠的行为痛反应由两个时相组成 :注射formalin后随即出现一个持续约 6～ 9min的第一相 ;在 9～ 15min有一个间歇期 ,大约从第 15min起又出现一个持续约 2 0～ 40min的第二相 ;并且在formalin注射后 2h处死动?
Physiological studies of pain perception have shown that nociceptive information is regulated at the spinal level by the spinal cord itself and the epistatic brain upon uploading. Numerous studies have also shown that the introduction of nociceptive information into the dorsal horn can cause the expression of immediate early genes (immediate early genes); c fos gene as one of the early genes, the fos protein produced in pain modulation Important role. Dynorphin A (dynorphin A, DynA) is mainly located in the dorsal horn interneurons, and mainly concentrated in the dorsal horn; in chronic inflammatory pain, spinal cord dynorphin content increased significantly, indicating that dynorphin in the spinal cord Horizontal pain information processing plays an important role, may be related to the plasticity of spinal pain feel. The purpose of this study was to detect the expression of fos protein in the dorsal horn by antisense oligodeoxynucleotide (AS ODN) technology to observe the relationship between Fos protein and slow pain reaction and to analyze the increase of fos protein production and the increase of DynA Expression, and to further explore the role of dynorphin A in pain modulation. Experiments were performed in Wistar rats (10 0 ~ 12 0 g). As a pre-experimental blank control group (n = 5), formalin (5%, 50 μl) was subcutaneously injected into the forelimb of the hindlimb at a time, The behavioral pain response consists of two phases: immediately after injection of formalin, a first phase lasting about 6 to 9 minutes, an intermittent phase at 9 to 15 minutes, and an approximately 20 to 40 minutes recurring from the 15th minute Of the second phase; and died at 2h after formalin injection?