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目的探讨5-氮杂胞苷(5-AZA)协同藤黄酸对K562/A02耐药细胞株的抑制作用。方法采用MTT比色法测定柔红霉素(DNR)的半数抑制量(IC50);流式细胞术(FCM)检测细胞凋亡;RT-PCR法检测mdr-1基因mRNA表达。结果 DNR对K562/A02的IC50为19.4 mg/L。5-AZA、藤黄酸和两药联合处理K562/A02细胞后,DNR的IC50值分别为11.1 mg/L、10.5 mg/L和7.1 mg/L。5-AZA与藤黄酸联合应用可增加K562/A02的凋亡。分别单独应用5-AZA、藤黄酸作用后耐药株mdr-1 mRNA下调微弱,两药联合作用后耐药株mdr-1 mRNA下调。结论 5-AZA、藤黄酸均能抑制耐药白血病细胞株K562/A02细胞,联合使用具有协同作用。
Aim To investigate the inhibitory effect of 5-azacytidine (5-AZA) cooperating with gambogic acid on K562 / A02 drug-resistant cell lines. Methods MTT assay was used to determine the half maximal inhibitory concentration (DN50) of daunorubicin (DNR). Cell apoptosis was detected by flow cytometry (FCM). The mRNA expression of mdr-1 was detected by RT-PCR. Results The IC50 of DNR to K562 / A02 was 19.4 mg / L. The IC50 values of DNR after treatment with 5-AZA, gambogic acid and two drugs were 11.1 mg / L, 10.5 mg / L and 7.1 mg / L, respectively. The combination of 5-AZA and gambogic acid can increase the apoptosis of K562 / A02. After treated with 5-AZA alone, the down-regulation of mdr-1 mRNA in drug-resistant strains was weak, and mdr-1 mRNA was down-regulated after the combination of the two drugs. Conclusions Both 5-AZA and gambogic acid can inhibit the K562 / A02 cell line, which has a synergistic effect.