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以过渡态理论为基础 ,以羧肽酶 A(CPA)的天然肽底物马脲酰 -苯丙氨酸为模型 ,分别以磷原子和硫原子为四面体过渡态类似物的中心原子来设计和合成了半抗原和全抗原 ,将两类抗原用免疫方法诱导制备出抗体 ,然后对两类抗体水解肽键的活性进行比较。
Based on the transition state theory, the natural peptide substrate of carboxypeptidase A (CPA) was used as a model, and the phosphorus atom and the sulfur atom were designed as the central atoms of tetrahedron analogs And the hapten and the whole antigen were synthesized. The two kinds of antigens were induced by the immunization method to prepare the antibodies, and then the activity of the two types of antibodies to hydrolyze the peptide bonds was compared.