论文部分内容阅读
背景:衰老是生物体内各组织、器官的退行性改变,是诸多病理、生理过程综合作用的结果。红景天是一种天然药用植物,具有延缓机体衰老、防治老年病等功能,但是其确切机制仍不十分清楚。目的:探讨藏药红景天提取物(诺迪康胶囊)抗衰老作用及其机制。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所军医大学医院的神经内科。材料:实验于2002-03/08在解放军第四军医大学药理学实验室完成。60只昆明种小鼠随机分为正常对照组、模型组、诺迪康1,2,4g/kg3个剂量组、维生素E阳性对照组0.1mg/kg,每组10只。干预:模型组、诺迪康组、维生素E阳性对照组均将30g/LD-半乳糖生理盐水溶液,按每日150mg/kg颈后部皮下注射,连续8周,同时,诺迪康组、维生素E阳性对照组灌胃给药,1次/d,连续8周。主要观察指标:诺迪康胶囊对衰老小鼠脑乳酸脱氢酶(lactatedehydroge-nase,LDH)活性、过氧化脂质(lipidperoxides,LPO)含量,脑过氧化物歧化酶(superoxidedismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量以及海马神经元线粒体超微结构的影响。结果:诺迪康胶囊1,2,4g/kg组均可降低脑组织LPO含量犤(30.1±2.9),(27.8±3.1),(26.9±1.9)nmol/g犦和MDA含量犤(110.2±13.5),(95.4±20.1),(90.2±16.5)nmol/g犦,与模型组犤(33.4
BACKGROUND: Senescence is the degenerative change of various tissues and organs in the organism, and is the result of a combination of many pathological and physiological processes. Rhodiola is a natural medicinal plant that has the functions of delaying body aging and preventing and treating senile diseases, but its exact mechanism is still not very clear. Objective: To investigate the anti-aging effects of Tibetan medicine Rhodiola rosea extract (Noritukang capsule) and its mechanism. Design: A randomized controlled experimental study of experimental animals. Unit: Neurology in a Military Medical University Hospital. MATERIALS: The experiment was performed at the Pharmacology Laboratory of the Fourth Military Medical University of Chinese PLA from March to August 2002. 60 Kunming mice were randomly divided into normal control group, model group, Nodicin 1, 2, 4g/kg 3 dose group, vitamin E positive control group 0.1mg/kg, 10 in each group. Intervention: The model group, Noriticon group, and Vitamin E positive control group all received a 30 g/L-galactose solution of saline in the back of the neck at a dose of 150 mg/kg per day for 8 consecutive weeks. At the same time, the Nordicon group, The vitamin E positive control group was administered intragastrically once a day for 8 weeks. MAIN OUTCOME MEASURES: The activity of lactate dehydrogenase (LDH), lipid peroxides (LPO), and superoxide dismutase (SOD) activity of Noritikang capsules in aging mice Malondialdehyde (MDA) content and ultrastructure of mitochondria in hippocampal neurons. RESULTS: In the 1,2,4 g/kg group of Noritikang capsules, the LPO content in brain tissue was decreased (30.1±2.9), (27.8±3.1), (26.9±1.9) nmol/g and MDA content (110.2±). 13.5), (95.4±20.1), (90.2±16.5) nmol/g犦, and the model group犤33.4