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以牛粪和甘蔗叶为堆肥原料,从实验室自制的强制通风发酵罐堆肥的降温阶段筛选出一株具有高效硝化作用的菌株N7。通过生理生化实验等方法对N7进行初步鉴定,验证并从pH、温度、转速、接种量及初始碳氮源浓度方面探究影响其硝化作用的条件。实验结果表明:菌株N7是一种可以利用亚硝酸盐转化成硝酸盐的细菌,在pH为3,温度为30℃,转速为140 r/min,接种量为1%,初始氮源浓度为0.2%,初始碳源浓度为0.05%时,其将亚硝酸根转化为硝酸根的效率达到最高。
Using cow dung and sugarcane leaves as composting materials, a strain N7 with high efficiency of nitrification was screened out from the cooling stage of forced air fermentor compost made in laboratory. Through physiological and biochemical experiments and other methods for preliminary identification of N7, verify and from the pH, temperature, speed, inoculum size and initial carbon and nitrogen concentration to explore the conditions affecting its nitrification. The results showed that strain N7 was a kind of bacterium which can convert nitrite into nitrate. The optimum conditions were as follows: pH 3, temperature 30 ℃, rotation speed 140 r / min, inoculum size 1%, initial nitrogen concentration 0.2 %. When the initial carbon source concentration is 0.05%, the efficiency of converting nitrite to nitrate is highest.