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[目的]研究麝香酮在体内试验条件下 ,对苯并(a)芘致小鼠肝和肺细胞核DNA损伤的影响。[方法]SENCAR雄性成年小鼠灌胃 ,分别单独给予苯并(a)芘125、250、500mg/(kg·bw·d)和用麝香酮250、500mg/(kg·bw·d)预处理后再给予苯并(a)芘125mg/(kg·bw·d) ,处死动物后分离小鼠肝和肺组织细胞核做彗星测试 ,采用CCD成像分析系统分析彗星 ,取尾相 (olivertailmoment,OTM)值判断DNA损伤强度。[结果]苯并(a)芘各浓度组均出现小鼠肝和肺细胞OTM值的明显增加 ,高浓度麝香酮[500mg/(kg·bw·d)]可引起小鼠肝细胞OTM值的轻度增加。用高浓度麝香酮预处理小鼠后再给予苯并(a)芘 ,所观察到的肝、肺的DNA损伤 (肝OTM值为7.6、肺为11)较之苯并(a)芘单独染毒引起的DNA损伤(肝OTM值为5.1、肺为6.9)增加更明显。[结论]麝香酮能明显增强苯并(a)芘所致的DNA损伤作用
[Objective] The research aimed to study the effect of musk ketone on the DNA damage of liver and lung in mice induced by benzo (a) pyrene in vivo. [Method] SENCAR male adult mice were intragastrically administrated with 125, 250, 500 mg / (kg · bw · d) benzopyrene and 250, 500 mg / (kg · bw · d) musk ketone respectively Then give 125 mg / (kg · bw · d) of benzo (a) pyrene. The animals were sacrificed and the nuclei of the liver and lung were isolated for comet assay. CCD imaging analysis system was used to analyze comet, olivertailment (OTM) Value to determine DNA damage intensity. [Result] The OTM value of liver and lung cells in each concentration of benzo (a) pyrene increased obviously. High concentrations of musk ketone [500mg / (kg · bw · d)] could cause the OTM value Mild increase. Benzo (a) pyrene was administered to mice pretreated with high concentrations of musk and DNA damage observed in the liver and lungs (hepatic OTM of 7.6 and lung of 11) compared with that of benzo (a) pyrene DNA damage caused by the virus (liver OTM value of 5.1, the lung is 6.9) increased more pronounced. [Conclusion] Musk ketone can obviously enhance DNA damage induced by benzo (a) pyrene