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本文报道了黄花菜的幼叶外植体接种在2.4—D的浓度为2—4 PPM的Ms培养基上,黑暗培齐,从切口处形成愈伤组织,将愈伤组织转入2PPM BA+0.5PPM IAA的Ms培齐基上分化成芽,再转入无激素Ms培养基培养,产生根,形成小苗。在上述附加2.4—D的Ms培养基上培养带花丝的花药,花丝基部膨大并形成不定芽,不定芽转入无激素Ms培养基上培养,形成大量胚状体。黄花菜组织培养中胚状体的获得,并大量繁殖,为组织培养作为黄花菜优良品种迅速繁殖的一种手段提供了可能性。
In this paper, we report that young explants of day-lily were inoculated on 2.4-D MS medium with a concentration of 2-4 PPM, and dark-cultivated. The callus was formed from the incision and the callus was transferred to 2PPM BA + 0.5PPM IAA’s Ms Pei Qi differentiation into buds, then transferred to hormone-free Ms medium culture, produce roots, the formation of seedlings. Anthers with filaments were cultured on Ms medium supplemented with 2.4-D as described above. The basal portions of the filaments were swollen and formed adventitious buds. Adventitious buds were transferred to hormone-free Ms medium to form a large number of embryoid bodies. Obtaining and multiplying embryoid bodies in lily tissue culture offers the possibility of tissue culture as a means of rapid propagation of elite good varieties.