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为测定我国肾综合征出血热疫苗生产株LR1株的全基因组序列 ,了解该株分子基础 ,从提取的细胞总RNA逆转录PCR扩增 ,产物纯化后克隆T载体纯化后测序 ,结果证明 ,LR1株全基因组序列由L6 5 33、M36 16、S片段的16 92个核苷酸组成 ,依各自读码框架分别编码 2 15 1、1135、42 9个氨基酸。序列同源比较分析表明 ,LR1毒株与国外HTN型毒株高度同源 ,属同一亚型 ,尤其与HTN代表株 76 - 1183个片段同源率高达 99 3%～ 99 8% ,而与国内的HTN型病毒差异较大 ,同源率仅为 79 4%～ 84 6 %。氨基酸比较也显示了同样的结果。
In order to determine the whole genome sequence of LR1 strain of China’s hemorrhagic fever with renal syndrome vaccine and to know the molecular basis of the strain, the total RNA of the strain was amplified by RT-PCR and the purified product was cloned and sequenced. The results showed that LR1 The complete genome sequence of the strain consisted of 1692 nucleotides of L6 5 33, M36 16 and S fragment, encoding 215, 1135 and 429 amino acids respectively according to their reading frames. Sequence homology analysis showed that the LR1 strain was highly homologous with HTN strains abroad, belonging to the same subtype, especially with homology of 99.3% ~ 99.8% with 76-1183 HTN strains, HTN virus, the homology rate is only 79 4% ~ 84 6%. Amino acid comparison also shows the same result.