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目的 进一步研究肝炎平在肝纤维化中的保护作用。方法 动物随机分为 5组 :正常组 (n =8) ,依肝炎平作用时间不同分设A、B、C组 (均n =15 ) ,D组为模型组 (n =15 )。肝炎平灌胃 1ml/(10 0g·次 ) ,1次 /d。模型组皮下注射CCl40 3ml/10 0 g体重 ,2次 /周 ,共用 9周。 结果 HE染色及电镜观察显示 :模型组肝脏失去正常结构 ,部分假小叶形成 ,而肝炎平组肝细胞脂肪变性和肝纤维化明显减少。模型组总胶原纤维面积 (84 0 2 3± 81 6 5 )μm2 及面积百分数 (7 0 0± 0 90 ) %明显增加 ,肝炎平组 (B组 )胶原纤维面积 (14 8 73± 4 5 89) μm2 及面积百分数(1 16± 0 33) % ,较模型组明显减少。模型组单胺氧化酶 (MAO)、酸性磷酸酶 (ACP)活性明显增加 ,琥珀酸脱氢酶 (SDH)活性明显下降。肝炎平组较模型组MAO、ACP活性明显下降 ,SDH活性明显增加。结论 肝炎平能减少氧自由基的释放 ,提高肝细胞的新陈代谢及免疫机能状态 ,有抗纤维化作用 ,是一种可应用于临床的安全、有效的药物。
Objective To further study the protective effect of hepatitis in liver fibrosis. Methods Animals were randomly divided into 5 groups: normal group (n = 8). A, B, and C groups (n = 15) were divided according to the time of action of Hepatitis A, and group D was a model group (n = 15). Hepatitis flat gavage 1ml/(10 0g·times), 1 time/d. The model group was injected subcutaneously with CCl40 3 ml/10 0 g body weight twice a week for 9 weeks. Results HE staining and electron microscopy showed that: in the model group, the liver lost its normal structure and some of the pseudo-lobules formed, while the hepatocyte steatosis and hepatic fibrosis were significantly reduced in the Hepatitis group. The total collagen fiber area (84 0 2 3± 81 65) μm 2 and area percentage (700 0 90 %) increased significantly in the model group, and the area of collagen fibers in the Hepatitis group (group B) was 14 8 73± 4 5 89. ) μm2 and area percentage (1 16 ± 0 33) %, significantly reduced compared with the model group. The activity of monoamine oxidase (MAO) and acid phosphatase (ACP) in the model group increased significantly, and the activity of succinate dehydrogenase (SDH) decreased significantly. Compared with the model group, the activity of MAO and ACP in the hepatitis group was significantly decreased, and the activity of SDH was significantly increased. Conclusion Hepatitis can reduce the release of oxygen free radicals, improve the metabolism and immune function of hepatocytes, and have antifibrotic effects. It is a safe and effective drug that can be used clinically.