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血清尿酸测定的标准参考方法是尿酸酶法(Unicase Method),该法系根据尿酸在紫外光293nm的吸光作用,当血清和缓冲的尿酸酶溶液在37℃保温一定时间后,由于尿酸酶的作用,尿酸被氧化为尿囊素(Allantoin),从而失去其吸光作用。因此,由吸光度减低的程度反映尿酸含量的变化,此法特异性高,但由于需要较贵重的仪器及试剂,目前在我国尚不可能广泛应用。近年国内文献有较简易方法介绍,如二氮杂菲铁直接显色法(1)和
The standard reference method for serum uric acid determination is the Unicase Method, which is based on the absorbance of uric acid at 293 nm UV light. When the serum and buffered uricase solution are incubated at 37 ° C. for a certain period of time, due to the action of uricase , Uric acid is oxidized to allantoin, losing its absorbency. Therefore, the decrease of absorbance reflects the change of uric acid content. This method has high specificity. However, due to the need of more expensive instruments and reagents, it is not yet widely available in our country at present. In recent years, domestic literature has a more simple method, such as diazo phenanthrene direct color method (1) and