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应用荧光分光光度法和放射免疫法 ,在以 6-羟基多巴胺 ( 6- OHDA)单侧损毁内侧前脑束 ( MFB)制备的偏侧帕金森病 ( PD)大鼠模型身上 ,测定了腹侧被盖区 ( VTA)和伏核 ( Acb)中多巴胺 ( DA)和八肽胆囊收缩素 ( CCK- 8)的含量 ,并观察了 VTA和 Acb区微量注射 CCK- 8对正常大鼠 DA含量的影响。结果如下 :PD大鼠模型损毁侧 VTA和 Acb的 DA和 CCK- 8的含量与健侧及对照组相比均减少 ( P<0 .0 1 ) ;而健侧 VTA和 Acb的 DA和 CCK- 8的含量同正常大鼠相比 ,差别无显著性 ( P>0 .0 5)。正常大鼠 Acb微量注射 CCK- 8能使 Acb的 DA含量增高 ( P<0 .0 5) ;而 VTA微量注射 CCK- 8则不影响 VTA和 Acb区的 DA含量 ( P>0 .0 5)。结果表明 ,6- OHDA单侧损毁 MFB后既降低中脑 DA能系统中 VTA和 Acb的 DA含量 ,又降低该系统中 VTA和 Acb区与 DA共存的 CCK- 8含量 ;并提示 Acb区外源性 CCK- 8可增加该区的 DA含量。
Fluorescent spectrophotometry and radioimmunoassay were used to determine the ventral telangiectasias (PD) in a rat model of hemiparkinsonism (PD) prepared by unilateral medial forebrain bundling (MFB) of 6-hydroxydopamine (6- OHDA) (DA) and octapeptide cholecystokinin (CCK-8) in the VTA and Acb, and the effect of CCK-8 injected into the VTA and Acb on the content of DA in normal rats influences. The results were as follows: The contents of DA and CCK-8 in the lesioned side of VTA and Acb in PD model rats were decreased compared with those in the contralateral and control groups (P <0.01), while DA and CCK- 8 content compared with normal rats, the difference was not significant (P> 0.05). Microinjection of CCK-8 into Acb in normal rats increased the content of DA in Acb (P <0.05), while microinjection of CCK-8 into VTA did not affect the DA content in VTA and Acb (P> 0.05) . The results showed that the unilateral destruction of MFB after 6-OHDA not only reduced the DA content of VTA and Acb in midbrain DA system, but also decreased the CCK-8 content of VTA and Acb area coexisting with DA in this system. Sex CCK-8 increases the DA content in this area.