论文部分内容阅读
为了从分子水平研究竹子光合作用的机理,采用RT-PCR及RACE方法从绿竹中分离了cab家族的一个基因,命名为BoLhca4-1(GenBank注册号为EF690303)。BoLhca4-1的cDNA序列全长931 bp,含有一个735 bp的开放阅读框,编码了一个244 aa的蛋白,分子量大小约为26.8 ku。序列分析结果表明,BoLhca4-1是光系统Ⅰ的一个捕光叶绿素复合蛋白基因,编码肽链与禾本科植物水稻的cab蛋白有着很高的同源性,达到86.1%。系统进化树分析表明,BoLhca4-1编码蛋白与水稻的cab蛋白亲缘关系较近。另外,对绿竹不同组织中BoLhca4-1基因的表达进行RT-PCR检测发现,其在叶片中的表达量较鞘和茎中要高。
In order to study the mechanism of bamboo photosynthesis at the molecular level, a gene of the cab family was isolated from Phyllostachys pubescens by RT-PCR and RACE and named BoLhca4-1 (GenBank accession number EF690303). The cDNA sequence of BoLhca4-1 is 931 bp in length and contains a 735 bp open reading frame encoding a 244 aa protein with a molecular weight of about 26.8 ku. Sequence analysis showed that BoLhca4-1 was a photosynthetic chlorophyll a complex protein gene of photosystem I. The coding protein chain shared 86.1% homology with the cab protein of gramineous rice. Phylogenetic tree analysis showed that the BoLhca4-1 protein has a close genetic relationship with the cab protein of rice. In addition, RT-PCR analysis of BoLhca4-1 gene expression in different tissues of Phyllostachys pubescens showed that its expression level in leaves was higher than that in sheath and stem.