本文采用L929细胞毒试验,在体外观察了三种微量元素锌、铜、硒对正常人外周血单个核细胞产生肿瘤坏死因子(TNF)的影响。结果发现锌、铜在体外能明显促进TNF产生,以0.25mmol/L为最适浓度,并能维持96小时以上。铜的作用较锌为强。硒虽然单独不能诱导TNF分泌,但与LPS协同能促使单个核细胞释放TNF。本文结果对研究上述三种元素参与免疫反应调节机理及TNF分泌调控均有裨益。 In this paper, L929 cytotoxicity assay was used to observe the effects of three trace elements, such as zinc, copper and selenium, on the production of tumor necrosis factor (TNF) in normal human peripheral blood mononuclear cells. The results showed that zinc and copper can significantly promote the production of TNF in vitro to 0.25mmol / L as the optimal concentration, and can maintain 96 hours or more. The role of copper is stronger than zinc. Although selenium alone can not induce TNF secretion, in synergy with LPS, mononuclear cells can release TNF. The results of this study on the three elements involved in the regulation of immune response and regulation of TNF secretion are beneficial.