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以３０例肺癌、４０例胃癌及２２例肝癌石蜡包埋组织提取的ＤＮＡ为模板，经两轮ＰＣＲ扩增一限制性内切酶酶切，用限制性酶切片段长度多态性方法（ＲＦＬＰ）检测ｃ－Ｋｉ－ｒａｓ第１２密码子点突变。结果发现，肺癌组织中２０％（６／３０）有突变，其中４０％（４／１０）鳞癌和２０％（２／１０）腺癌有突变，小细胞肺癌（０／１０）无突变；４０例胃癌中仅１例（２．５％）有突变，２２例肝癌无１例突变。ＰＣＲ－ＲＦＬＰ是一种很敏感及快速检测基因突变的方法。
The DNA extracted from paraffin-embedded tissues from 30 lung cancers, 40 gastric cancers and 22 hepatocellular carcinomas was used as a template and amplified by restriction endonuclease digestion in two rounds of PCR using restriction fragment length polymorphism (RFLP). ) Detection of c-Ki-ras point mutation at codon 12. The results showed that 20% (6/30) of lung cancer tissues had mutations, of which 40% (4/10) of squamous cell carcinomas and 20% (2/10) of adenocarcinomas had mutations, and small cell lung cancer (0/10) had no mutations. Among 40 cases of gastric cancer, only one case (2.5%) had mutations, and 22 cases of liver cancer did not have one case of mutation. PCR-RFLP is a very sensitive and rapid method for detecting gene mutations.