论文部分内容阅读
目的:以桦纤孔菌为材料,研究不同矿质元素(钙、铁、锌)在其菌丝体中的富集及其对生长的影响,并探究富集矿质元素对桦纤孔菌总三萜含量的影响。方法:对桦纤孔菌进行矿质元素富集,并对总三萜的提取、测定方法进行优化,测定富集矿质元素的桦纤孔菌菌丝中总三萜物质含量。结果:在PDA培养基中添加1 000 mg/L的Ca(NO_3)_2能明显促进菌丝体生长和产量提高,钙的富集率可高达0.07%;最适宜的铁源是Fe_2(SO_4)_3,添加浓度为300 mg/L,铁的富集率最高为0.29%;当添加Zn SO4浓度为175 mg/L的处理菌丝长势最强,锌的富集率最高可达0.57%。富集钙元素的桦纤孔菌中总三萜含量最高,可达0.058 mg/m L,比对照提高5.45%;富集铁的桦纤孔菌总三萜含量最低,仅为0.04 mg/m L。结论:富集矿质元素钙的桦纤孔菌中总三萜含量较高,该研究为桦纤孔菌工厂化生产开辟了新途径并提供了基础数据。
Objective: To study the enrichment of different mineral elements (Ca, Fe, Zn) in mycelium and their effects on the growth of Betula platyphylla, Effect of terpene content. Methods: The mineral elements were enriched in Pseudomonas fluorescens, and the total triterpenoids were extracted and determined. The content of total triterpenoids in the mycelia of Pseudotrichthora massoniana enriched in mineral elements was determined. Results: Adding 1 000 mg / L Ca (NO_3) _2 to PDA medium could significantly promote mycelial growth and yield, and the enrichment rate of calcium could reach as high as 0.07%. The most suitable iron source was Fe_2 (SO_4) L, the highest enrichment rate of iron was 0.29%. When the concentration of ZnSO4 was 175 mg / L, the treated mycelium grew the most, and the enrichment rate of Zn was up to 0.57%. The content of total triterpene in Caf-rich bacteria was the highest (0.058 mg / m L), which was 5.45% higher than that of the control. The total triterpene content of iron-rich Betulaceae was only 0.04 mg / m L. Conclusion: The content of total triterpenoids in the betula of Betula platyphylla enriched in mineral elemental calcium is high. This study opens up new ways and provides basic data for industrial production of Betula platyphylla.