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目的用NaCl模拟盐胁迫,研究接种内生真菌对菊花抗盐特性的影响。方法采用盆栽试验,以与内生真菌葡萄孢(C1菌株)、球毛壳菌(C4菌株)共生培养的菊花为材料,研究不同浓度盐胁迫对不同处理组菊花生理指标的影响。结果各处理组菊花根叶的含水量随着盐分胁迫的加重而降低,接菌处理组的根叶含水量减失程度比对照组小。各处理组SOD酶活力、可溶性蛋白量均随NaCl浓度提高而增加,20 g/L NaCl时均达到最大值,接菌处理组SOD酶活力、可溶性蛋白量高于对照组,C4组高于C1组。POD酶活力均先升高后降低,15 g/L NaCl时各处理组POD酶活力达到最大值,C4、C1组POD酶活力分别比对照组高25.50%、1.35%。15 g/L NaCl处理时,C4组PAL活力约是对照组的7倍。结论内生真菌增加了菊花的抗盐能力,C4组效果好于C1组。
Aim To study the effects of inoculation endophytic fungi on the salt-tolerance of chrysanthemum in salt stress. Methods The pot experiment was conducted to study the effects of salt stress on the physiological indexes of chrysanthemum in different treatments with the endophytic fungi Botrytis cinerea (C1) and Chaetomium globosum (C4). Results The water content of root and leaf of chrysanthemum decreased with the increase of salt stress in each treatment group. The reduction of water content in roots and leaves of inoculation group was less than that in control group. The activities of SOD and soluble protein in all treatment groups increased with the increase of NaCl concentration, and reached the maximum at 20 g / L NaCl. The activities of SOD and soluble protein in the treated groups were higher than those in the control group, while those in C4 group were higher than those in C1 group. POD activity increased first and then decreased. POD activity in each treatment group reached the maximum at 15 g / L NaCl, and POD enzyme activities in the C4 and C1 groups were 25.50% and 1.35% higher than that of the control group respectively. At 15 g / L NaCl treatment, the PAL activity of C4 group was about 7 times of that of control group. Conclusion Endophytic fungi increased the salt tolerance of chrysanthemum, and the effect of C4 group was better than that of C1 group.