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我们观察了蜂毒对大鼠实验性肝纤维化的作用及其毒副作用,以期对人的肝纤维化及肝硬化的治疗有所借鉴。材料与方法一、实验动物:体重160～210g 雄性 SD 大鼠,由中国医学科学院实验动物研究所购进。二、药物:精制蜂毒注射液,0.5mg/ml。三、预备实验:以35mg/100ml 的苯巴比妥钠溶液代替常水,喂饲动物10天。四、CCl_4损伤性肝纤维化模型:以每克鼠体重0.5μl增减,确定给予 CCl_4量,每周1次,每次最大量不超过250μl。五、动物分组:随机分成6组,每组24只大鼠。①模型组。②正常对照组。③蜂毒10μg治疗组:在肝纤维化模型制备完成后开始给药,每天10μg腹腔注射,每周6次共3个月。④蜂毒20μg治疗组:剂量及给药方法同③组。⑤蜂毒10μg预防组:实验开始即给药,给药剂量及方法同③组共6个月。⑥蜂毒药物
We observed the effect of bee venom on experimental hepatic fibrosis and its toxic and side effects in rats in order to learn from the treatment of human liver fibrosis and cirrhosis. Materials and methods First, experimental animals: 160 ~ 210g male SD rats purchased from the Institute of Experimental Animals, Chinese Academy of Medical Sciences. Second, drugs: refined bee venom injection, 0.5mg / ml. Third, the preparatory experiment: 35mg / 100ml phenobarbital sodium solution instead of Changshui, feeding animals for 10 days. Fourth, CCl_4 injury of liver fibrosis model: 0.5g per gram of body weight changes, to determine the amount of CCl_4, once a week, each time the maximum does not exceed 250μl. Five animal groups: randomly divided into 6 groups, each group of 24 rats. ① model group. ② normal control group. ③ Bee venom 10μg treatment group: The liver fibrosis model was prepared after the start of administration, 10μg intraperitoneal injection, 6 times a week for a total of 3 months. ④ bee venom 20μg treatment group: dose and administration method with the ③ group. ⑤ bee venom 10μg prevention group: the beginning of the experiment that is administered, the dose and method of administration with the ③ group of 6 months. ⑥ bee venom drugs