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目的建立反相高效液相-紫外检测法测定人血浆中的头孢美唑浓度。方法内标法定量,以头孢唑啉为内标,采用YMC-C18(150mm×4.6mm,5μm)柱,以0.1mol/L Na Ac(冰醋酸调p H=6.88):乙腈=85:15为流动相,流速0.9m L/min,柱温为室温,紫外检测波长为272nm,直接沉淀蛋白后反洗进样。结果标准曲线线性范围为:0.25~100μg/m L,线性=0.99995;最低定量限为0.25μg/m L;预处理回收率为:76.48%~99.65%;方法回收率为99.84~104.73%;日内RSD在0.68%~1.83%之间,日间RSD在2.06%~6.40%之间。结论该方法操作简单,结果准确,重现性好,可用于人血浆中的头孢美唑的含量测定和药动学研究。
Objective To establish an RP-HPLC method for the determination of cefmetazole in human plasma. Methods The internal standard method was used. The internal standard was cefazolin, using YMC-C18 (150mm × 4.6mm, 5μm) column with 0.1mol / L Na Ac As the mobile phase, the flow rate was 0.9m L / min, the column temperature was room temperature, UV detection wavelength was 272nm, the protein was directly precipitated backwash injection. Results The linear range of standard curve was 0.25 ~ 100μg / m L and linearity was 0.99995. The lowest limit of quantification was 0.25μg / m L. The recoveries of pretreatment ranged from 76.48% to 99.65%. The recoveries were 99.84% ~ 104.73% RSD ranged from 0.68% to 1.83%, and intraday RSD ranged from 2.06% to 6.40%. Conclusion The method is simple, accurate and reproducible. It can be used in the determination of cefmetazole and pharmacokinetics in human plasma.