论文部分内容阅读
为查清免疫促进剂对日本蟳(Charybdis japonica)(俗称石蟹)自身免疫力的增强作用及机理,对脂多糖(LPS)、β-葡聚糖(-β1,3-glucan)、灭活鳗弧菌和灭活哈维氏弧菌对日本蟳酚氧化酶(PO)的产量与活性以及血细胞的数量与超微结构的影响进行了研究。结果表明,经4种免疫促进剂处理后,日本PO产量和总酶活性都有显著增加,但PO的比活性与对照相比没有显著差异。日本蟳血细胞超微结构观察显示,多糖处理组小颗粒细胞和大颗粒细胞的糙面内质网和线粒体数量增加,颗粒数量减少、体积增大,而透明细胞的超微结构没有显著变化;灭活弧菌处理组小颗粒细胞中的颗粒数量明显减少,透明细胞中线粒体的数量显著增加。由此可见,多糖类和灭活弧菌类免疫促进剂对日本非特异性免疫系统的影响不同,多糖类免疫促进剂主要提高酚氧化酶原激活组分PO的产量,进而提高PO总酶活性,而灭活弧菌类免疫促进剂主要提高透明细胞和小颗粒细胞的吞噬活性。
In order to investigate the enhancing effect and mechanism of immunostimulants on the autoimmunity of Charybdis japonica (Crab), the effects of lipopolysaccharide (LPS), β-glucan (β-glucan), inactivated eel The effects of Vibrio and Vibrio harveyi on the production and activity of cortisol oxidase (PO) and the number and ultrastructure of blood cells were studied. The results showed that after the four kinds of immunostimulants were treated, PO yield and total enzyme activity in Japan increased significantly, but the specific activity of PO was not significantly different from the control. The ultrastructure observation of Japanese blood cells showed that the numbers of rough endoplasmic reticulum and mitochondria of small granulosa cells and large granulocytes increased, the number of particles decreased, the volume increased, but the ultrastructure of clear cells did not change significantly; The number of small particles in Vibrio-treated group was significantly reduced, and the number of mitochondria in clear cells was significantly increased. Thus, polysaccharides and inactivated Vibrio immunosuppressants have a different impact on non-specific immune system in Japan. Polysaccharide immunostimulants mainly increase the production of PO, a prooxidative enzyme, and then increase the activity of PO total enzyme Activity, and inactivated Vibrio immunostimulants mainly increase the phagocytic activity of clear cells and small granulocytes.