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目的 探讨高浓度氧 (简称高氧 )对早产鼠肺一氧化氮合酶 (NOS)分布及基因表达的影响。方法 将 2 1d孕早产鼠随机分为高氧暴露组 (简称高氧组 )和空气对照组 (对照组 ) ,分别置于常压高氧仓中 (氧体积分数 >0 95 )和正常空气中暴露 7d ,采用逆转录 聚合酶链反应 (RT PCR)、免疫蛋白分析和免疫组织化学染色观察NOS分布及基因表达。此外对肺组织干 /湿重比值 ,支气管肺泡灌洗液 (BALF)成份和肺病理组织变化也进行了对比分析。结果 高氧组与对照组比较 ,早产鼠肺组织有明显水肿、出血和炎症 ;高氧组支气管肺泡灌洗液中蛋白含量 (中位数为 1 4 9g/L)、细胞数 (中位数为 139 70× 10 7/L)和肺组织干 /湿重比值 (5 5 7± 0 2 9)较对照组 (分别为 :0 32g/L、16 30× 10 7/L、5 2 9± 0 2 5 )均明显增加 (t=2 8、2 1、2 2 9,P均 <0 0 5 ) ;高氧组肺组织内皮细胞型一氧化氮合酶 (eNOS)mRNA、eNOS和诱导型一氧化氮合酶 (iNOS)蛋白表达 (分别为 :1 0 2± 0 0 6、8 77± 0 75、4 6 1± 0 6 5 )较对照组 (分别为 :0 70± 0 12、4 5 2± 1 0 2、3 2 4± 0 5 5 )明显增加 (t =6 36、8 14、3 2 1,P <0 0 1、P <0 0 1、P <0 0 5 )。iNOSmRNA也显示有增加的趋势 ,但差异无统计学意义 ,同时免疫组织化
Objective To investigate the effect of high concentration oxygen (Hyperoxia) on the distribution and gene expression of lung nitric oxide synthase (NOS) in premature rats. Methods 21 premature pregnant rats were randomly divided into two groups: hyperoxia group (control group) and air control group (control group), which were placed in normal pressure hyperbaric oxygen chamber (oxygen volume fraction> 0 95) and normal air Seven days after exposure, NOS distribution and gene expression were observed by reverse transcription polymerase chain reaction (RT PCR), immunohistochemical analysis and immunohistochemical staining. In addition, lung tissue dry / wet weight ratio, bronchoalveolar lavage fluid (BALF) composition and lung pathological changes were also analyzed. Results Compared with the control group, the hyperoxia group showed obvious edema, hemorrhage and inflammation in the lung tissue of premature rats. The content of protein in the bronchoalveolar lavage fluid in the hyperoxia group (median 1449g / L), cell number (median (139 70 × 10 7 / L) and lung tissue dry / wet weight ratio (57 57 ± 0 2 9) compared with the control group (0 32 g / L, 16 30 × 10 7 / L, 5 2 9 ± 0 2 5) were significantly increased (t = 2 8,2 1,2 2 9, P <0 05). The expression of eNOS mRNA, eNOS and inducible The expression of iNOS protein was higher than that of the control group (0 70 ± 0 12 and 4 respectively 5 2 ± 10 23 2 4 ± 0 5 5) (t = 6 36,8 14,3 2 1, P <0.01, P <0.01, P <0.05). iNOS mRNA also showed an increasing trend, but the difference was not statistically significant, while immunohistochemistry