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背景与目的:肿瘤细胞靶向的细胞因子基因治疗是肿瘤治疗领域的研究热点。γ干扰素通过多种机制发挥抗肿瘤免疫的作用。本研究探讨利用γ-干扰素(IFN-γ)基因治疗对大肠癌的预防和治疗作用。方法:利用BALB/C小鼠成瘤的小鼠结肠癌CT26细胞株制备小鼠结肠癌腹腔转移瘤模型,用携带鼠IFN-γ基因的重组缺陷型腺病毒AdIFN-γ进行治疗,同时利用携带LacZ(β-galactosidase)基因的腺病毒AdLacZ和PBS(phosphate-bufferedsaline)作空白对照,检测经基因治疗后小鼠体内IFN-γ基因的表达情况、脾脏的细胞毒性T淋巴细胞(CTL)活性变化、肝转移的发生及荷瘤小鼠的生存期。结果:经IFN-γ基因治疗后,与对照组相比,治疗组小鼠血清中IFN-γ表达量明显增加(P<0.01),脾脏的CTL活性明显增强(P<0.05),肿瘤生长受到抑制,肝转移的发生率明显下降,荷瘤小鼠的存活期明显延长。结论:利用IFN-γ基因治疗大肠癌具有明显的疗效,并对其肝转移具有一定的预防作用。
Background and Objective: Cytokine gene therapy targeting tumor cells is a hot topic in the field of cancer therapy. Interferon gamma exerts antitumor immunity through a variety of mechanisms. This study explored the use of interferon-γ (IFN-γ) gene therapy for the prevention and treatment of colorectal cancer. Methods: The model of peritoneal metastasis of mouse colon cancer was established by using CT26 cell line of mouse colon carcinoma of BALB / C mice. The model was treated with AdIFN-γ recombinant adenovirus carrying murine IFN-γ gene. LacZ (β-galactosidase) adenovirus AdLacZ and PBS (phosphate-bufferedsaline) as a blank control to detect the gene expression of IFN-γ gene in mice after treatment, cytotoxic T lymphocytes (CTL) activity changes , The occurrence of liver metastasis and the survival of tumor-bearing mice. Results: Compared with the control group, the IFN-γ expression in the treated group was significantly increased (P <0.01) and the CTL activity in the spleen was significantly increased (P <0.05) Inhibition, the incidence of liver metastases decreased significantly, tumor-bearing mice significantly prolonged survival. Conclusion: The treatment of colorectal cancer with IFN-γ gene has obvious curative effect, and it has certain preventive effect on liver metastasis.