【摘 要】
：
Aflatoxins are potent carcinogens,mutagens and teratogens,and are harmful to both humans and animals.As many as 30 genes are involved in aflatoxin biosynthesis.Among them,aflM (ver-1) gene was predicted to encode a 28-kDa NADPH-dependent ketoreductase (AF
【机 构】
：
No.1 Senior High School of Wuhan Economic Development Zone,Wuhan,430056,China;Oil Crops Research Ins
论文部分内容阅读
Aflatoxins are potent carcinogens,mutagens and teratogens,and are harmful to both humans and animals.As many as 30 genes are involved in aflatoxin biosynthesis.Among them,aflM (ver-1) gene was predicted to encode a 28-kDa NADPH-dependent ketoreductase (AFLM),which catalyzed middle enzymatic steps in aflatoxin biosyn-thetic pathway.AFLM (Ver-1) was proved to be necessary for conversion of versicolorin A (VERA) to deme-thylsterigmatocystin (DMST) in aflatoxin B1 (AFB1) biosynthesis.For these reasons,aflM gene was cloned and specific monoclonal antibodies for AFLM was developed to better define potential pathways of AFLM involved in AFB1 biosynthesis.Monoclonal antibodies 11B2-1D7 and 3G5-4E7 were successfully screened out by immunizing mouse.Immunoblot analysis revealed that both had high sensitivity and specificity to identify native AFLM protein in A.flavus with detection limit of 11 ng/mL and 8 ng/mL respectively.These results showed that it was suitable for quantitative detection of AFLM in A.flavus isolate.Further investigation revealed that aflatoxin ac-cumulations of various A.flavus were not dependent on AFLM biosynthesis.Overall,this is the first report for development for AFLM monoclonal antibody development and application in A.flavus quantitative detection.
其他文献
对于髌骨脱位的治疗,目前主要通过临床检查来确诊脱位的等级,再根据等级的不同来选择治疗方案,临床上对于髌骨脱位主要分为4个等级,对于小型犬Ⅰ、Ⅱ级可通过保守治疗,Ⅲ级以上则需要手术治疗,对于大型犬来说,因为运动量和身体负重等原因在Ⅱ级以上就需要手术治疗[1].选择治疗方案和手术方案时会根据实际的病例情况优化治疗方案,从而提高治愈率,降低复发率和并发症.目前,临床上治疗犬髌骨脱位的手术方法主要选择滑车沟加深术,而选择用髌骨滑车沟置换术(Patellar groove replacement,PGR)治疗髌骨脱
本试验收集临床白臀长尾猴(Cercopithecus neglectus)血液标本9份,分别使用全自动血液分析仪和人工显微镜检法对红细胞、白细胞、血红蛋白含量进行计数检测,并对白细胞进行分类分析,初步统计白臀长尾猴的血液生理指标,同时对人工计数和仪器计数获得的数据进行差异显著性和相关性分析.结果 表明,通过两种方法获得的红细胞计数(r =0.006)、白细胞计数(r=0.000)、血红蛋白含量、淋巴细胞比率(r=0.000)、中性粒细胞比率(r=0.000)、单核细胞比率(r=0.096)结果相关性均较好
瘫痪是指骨骼肌随意运动能力减弱或丧失.后躯瘫痪俗称“腰痿”,主要病状为腰部僵硬,后躯运动不灵活,局部反应性降低,不愿起立,若强迫行走,则运步迟滞.轻度麻痹时,预后良好;若陷入严重麻痹,预后慎重[1].引起犬后躯瘫痪的原因有很多:肥胖压迫神经、外力作用引起脊髓挫伤、椎间盘突出致脊髓受压等,常发于贵宾、京巴、腊肠、比格等小型犬[2].
为了给东北虎细小病毒感染病例的临床诊断和治疗提供借鉴,本试验通过临床诊断、血常规检查、病原PCR检测、影像学检查、组织器官剖检、病理组织切片观察等方法,分析1例东北虎细小病毒群发感染病例,并采用抗病毒、调节免疫、止血止吐对症治疗、控制继发感染、营养支持和调节电解质平衡等疗法对该东北虎及其他患病东北虎大群实施治疗.结果 表明,通过及时确诊、综合治疗、细心护理,能促进感染细小病毒的东北虎群尽早恢复正常,病情得到有效控制.
淋证的主要症状表现为小便频急、淋漓不尽、尿道涩痛、小腹拘急、痛引腰腹等,类似于西医概念中的急、慢性尿路感染,泌尿系结石,前列腺炎,膀胱炎,乳糜尿等疾病的症状.淋证最早见于黄帝内经《素问.六元正纪大论》中,“淋者,淋漓不尽,如雨淋而下;閟,通秘,不通之意也.”指出了淋证既有可能是小便淋漓不畅,又有可能是闭阻不通之象.淋证主要由情志失调、饮食不节、禀赋不足、劳伤过度、久病缠身等因素引起,造成湿热蕴结下焦,肾与膀胱气化不利[1].
Low temperature coupled with high soil moisture during sowing to emergence generally results in poor peanut stand,thereby posing a non-negligible threat to peanut production in north and northeast China.Five high-oleic(HO) peanut cultivars and 4 seed dres
Alpha linolenic acid (ALA) is an essential polyunsaturated fatty acids that can improve human health.Rapeseed is the second largest oil crop in the world but the ALA content in its seed fatty acids is only about 10％.Two rapeseed germplasms YH25005 and R8Q
High-oleic peanuts has been recognized by processing sectors,seed sellers and consumers for their longer shelf life,longer seed life and mutiple healthe benefits.High oleate is becoming a requisite for varietal releases in many peanut breeding programs at
皮肤无力(Cutaneous asthenia),同义词Ehlers-Danlos综合征,是一种罕见的因遗传性胶原蛋白生成障碍引起的结缔组织发育不全综合征.患病特点为幼年发病,皮肤过于脆弱和过度伸展,皮肤极易撕裂.由于患猫精神、体格、血常规和生化等均正常,表现出的症状以皮肤抓伤为主,容易被诊断为普通的皮肤病和外伤,延误治疗和松懈管理,导致频繁受伤.可通过病史、伤口特点、伤口愈合进程、皮肤延展性指数和组织病理学检查等作出诊断.暂无明确有效的治愈方法,良好的管理可以使患猫达到正常寿命,目前国内该病的病例报道罕
The International Symposium on Lipid Science and Health (ISLSH),which was organized annually by Oil Crops Research Institute of Chinese Academy of Agricultural Sciences (OCRI-CAAS) since 2016,has gained a strong reputation and attracted hundreds of delega