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目的:研究紫花牡荆素诱导人肝癌HepG2细胞分化作用。方法:体外培养人肝癌Hep G2细胞,采用瑞氏-姬姆萨染色显微镜下测量细胞核质比,放射免疫法检测细胞上清液甲胎蛋白(AFP)水平,酶促反应试剂盒检测细胞碱性磷酸酶(ALP)和γ-谷胺酰转肽酶(γ-GT)的活性;Diamond stone分光光度法测定细胞酪氨酸α-酮戊二酸转氨酶(TAT)的活性。结果:1.0μmol/L紫花牡荆素和全反式维甲酸处理48 h,人肝癌Hep G2细胞核质比降低;AFP的分泌和γ-GT的活性显著降低(P<0.01);ALP和TAT的活性则显著升高(P<0.01)。结论:紫花牡荆素具有诱导人肝癌Hep G2细胞分化作用。
Objective: To study the differentiation of HepG2 cells induced by Vitexin. Methods: Hep G2 cells were cultured in vitro. The ratio of nucleus to cytoplasm was measured by Wright-Giemsa staining. The level of AFP was detected by radioimmunoassay. The enzymatic reaction kit was used to detect the alkaline Activity of phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT); and diamond stone spectrophotometry was used to determine the activity of tyrosine α-ketoglutarate transaminase (TAT). Results: After treatment with 1.0μmol / L Vitexin and all-trans retinoic acid for 48 h, the ratio of nuclear to cytoplasm of Hep G2 cells was decreased; the secretion of AFP and the activity of γ-GT were significantly decreased (P <0.01); ALP and TAT Activity was significantly increased (P <0.01). Conclusion: Vitexin can induce the differentiation of human Hep G2 cells.