论文部分内容阅读
AIM: To investigate associations between the tumor necrosis factor alpha(TNF-α)-1031 T>C,-863 C>A,-857 C>T,-308 G>A,and-238 G>A polymorphisms and HCC in Korea.METHODS: Hepatocellular carcinoma(HCC) cases were diagnosed at CHA Bundang Medical Center from June 1996 to August 2008. The association between TNF-α polymorphisms and HCC was analyzed in 157HCC patients and 201 controls using a polymerase chain reaction-restriction fragment length polymorphism assay. We investigated five TNF-α polymorphisms,which are TNF-α-1031 T>C,-863 C>A,-857 C>T,-308 G>A,and-238 G>A. The TNF-α genotype frequencies,genotype combinations and haplotypes were analyzed to disclose the association with HCC.RESULTS: None of the TNF-α polymorphisms was significantly associated with HCC. However,nine genotype combinations had associations with increased likelihood of HCC. Among them,TNF-α-1031/-857/-238 TT/CC/GA(AOR = 18.849,95%CI: 2.203-161.246,P = 0.007),TNF-α-1031/-308/-238 TT/GG/GA(AOR = 26.956,95%CI: 3.071-236.584,P = 0.003),and TNF-α-1031/-238 TT/GA(AOR = 21.576,95%CI: 2.581-180.394,P = 0.005) showed marked association with HCC. There were five haplotypes of TNF-α polymorphisms which were significantly associated with HCC. They are TNF-α-1031/-863/-857/-308/-238 T-C-C-G-A(OR = 25.824,95%CI: 1.491-447.223,P = 0.0005),TNF-α-1031/-857/-308/-238 T-C-G-A(OR = 12.059,95%CI: 2.747-52.950,P < 0.0001),TNF-α-1031/-857/-238 T-C-A(OR = 10.696,95%CI: 2.428-47.110,P = 0.0001),TNF-α-1031/-308/-238 T-G-A(OR = 7.556,95%CI: 2.173-26.280,P = 0.0002) and TNF-α-1031/-238 T-A(OR = 10.865,95%CI: 2.473-47.740,P = 0.0001). Moreover,HCC Okuda stage Ⅲ cases with the TNF-α-1031 CC genotype had better survival than those with the TT genotype(AOR = 5.795,95%CI: 1.145-29.323). CONCLUSION: Although no single TNF-α polymorphism is associated with HCC in this study,some TNF-α genotype combinations and haplotypes are associated with HCC. In addition,HCC Okuda stage Ⅲ cases with the TNF-α-1031 TT genotype may have a better prognosis than those with the CC genotype.
AIM: To investigate associations between the tumor necrosis factor alpha (TNF-α) -1031 T> C, -863 C> A, -857 C> T, -308 G> A, and-238 G> A polymorphisms and HCC in Korea. METHODS: Hepatocellular carcinoma (HCC) cases were diagnosed at CHA Bundang Medical Center from June 1996 to August 2008. The association between TNF-α polymorphisms and HCC was analyzed in 157HCC patients and 201 controls using a polymerase chain reaction-restriction fragment length polymorphism assay. We investigated five TNF-α polymorphisms, which are TNF-α-1031 T> C, -863 C> A, -857 C> T, -308 G> A, and-238 G> α genotype frequencies, genotype combinations and haplotypes were analyzed to disclose the association with HCC .RESULTS: None of the TNF-α polymorphisms was significantly associated with HCC. However, nine genotype combinations had associations with increased likelihood of HCC. Among them, TNF- (AOR = 18.849, 95% CI: 2.203-161.246, P = 0.007), TNF-α-1031 / -308 / -238 TT / GG / GA = 26.956, 95% CI: 3. There were five haplotypes of TNF (071-236.584, P = 0.003), and TNF-α-1031 / -238 TT / GA -α polymorphisms which were significantly associated with HCC. They are TNF-α-1031 / -863 / -857 / -308 / -238 TCCGA (OR = 25.824,95% CI: 1.491-447.223, P = 0.0005) α-1031 / -857 / -308 / -238 TCGA (OR = 12.059,95% CI: 2.747-52.950, P <0.0001) TNF-α-1031 / -308 / -238 TGA (OR = 7.556, 95% CI: 2.173-26.280, P = 0.0002) and TNF-α-1031 / -238 TA (OR = 10.865, 95% CI: 2.473-47.740, P = 0.0001). Moreover, HCC Okuda stage III cases with the TNF-α-1031 CC genotype had better survival than those with the TT genotype (AOR = 5.795, CI: 1.145-29.323). CONCLUSION: Although no single TNF-α polymorphism is associated with HCC in this study, some TNF-α genotype combinations and haplotypes are associated with HCC. In addition, HCC Okuda stage Ⅲ cases with the TNF- α -1031 TT genoty pe may have a better prognosis than those with the CC genotype.